Human BCL11A ELISA Kit

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  • Alternative name

    Human BCL-11A ELISA Kit;Human B-cell CLL/lymphoma 11A ELISA Kit;Human COUP-TF-interacting protein 1 ELISA Kit;Human ecotropic viral integration site 9 protein homolog ELISA Kit;Human EVI-9 ELISA Kit;Human zinc finger protein 856 ELISA Kit;Human CTIP1 ELISA Kit;Human EVI9 ELISA Kit;Human KIAA1809 ELISA Kit;Human ZNF856 ELISA Kit;Human BCL11A-L ELISA Kit;Human BCL11A-S ELISA Kit;Human BCL11A-XL ELISA Kit;Human BCL11a-M ELISA Kit;Human DILOS ELISA Kit;Human HBFQTL5 ELISA Kit;Human B-cell lymphoma/leukemia 11A ELISA Kit;Human B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2 ELISA Kit;Human BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1 ELISA Kit;Human C2H2-type zinc finger protein ELISA Kit;Human ecotropic viral integration site 9 homolog ELISA Kit;

  • Catalog
    E004320
  • species
    Human
  • GeneBCL11A
  • Standard CurveHuman BCL11A ELISA Kit
  • Other Species Mouse Bcl11a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BCL11A. No significant cross-reactivity or interference between BCL11A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL11A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman BCL11A ELISA Kit allows for the in vitro quantitative determination of BCL11A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: BCL11A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL11A antibody and an BCL11A-HRP conjugate. The assay sample and buffer are incubated together with BCL11A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL11A concentration since BCL11A from samples and BCL11A-HRP conjugate compete for the anti-BCL11A antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL11A from the sample, fewer sites are left to bind BCL11A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL11A concentration in each sample is interpolated from this standard curve.
  • Human B-cell lymphoma/leukemia 11A Protein information
  • Uniprot ID BC11A_HUMAN
  • Uniprot AC Q9H3G9; Q9NWA7;
  • UniGene Hs.370549;
  • GeneID 53335
  • KEGG hsa:53335;
  • Human B-cell lymphoma/leukemia 11A Protein SEQUENCE
  • SEQUENCE 835 AA; 91197 MW; D36A7D0BE6976DCF CRC64;

    MSRRKQGKPQ HLSKREFSPE PLEAILTDDE PDHGPLGAPE GDHDLLTCGQ

    CQMNFPLGDI LIFIEHKRKQ CNGSLCLEKA VDKPPSPSPI EMKKASNPVE

    VGIQVTPEDD DCLSTSSRGI CPKQEHIADK LLHWRGLSSP RSAHGALIPT

    PGMSAEYAPQ GICKDEPSSY TCTTCKQPFT SAWFLLQHAQ NTHGLRIYLE

    SEHGSPLTPR VGIPSGLGAE CPSQPPLHGI HIADNNPFNL LRIPGSVSRE

    ASGLAEGRFP PTPPLFSPPP RHHLDPHRIE RLGAEEMALA THHPSAFDRV

    LRLNPMAMEP PAMDFSRRLR ELAGNTSSPP LSPGRPSPMQ RLLQPFQPGS

    KPPFLATPPL PPLQSAPPPS QPPVKSKSCE FCGKTFKFQS NLVVHRRSHT

    GEKPYKCNLC DHACTQASKL KRHMKTHMHK SSPMTVKSDD GLSTASSPEP

    GTSDLVGSAS SALKSVVAKF KSENDPNLIP ENGDEEEEED DEEEEEEEEE

    EEEELTESER VDYGFGLSLE AARHHENSSR GAVVGVGDES RALPDVMQGM

    VLSSMQHFSE AFHQVLGEKH KRGHLAEAEG HRDTCDEDSV AGESDRIDDG

    TVNGRGCSPG ESASGGLSKK LLLGSPSSLS PFSKRIKLEK EFDLPPAAMP

    NTENVYSQWL AGYAASRQLK DPFLSFGDSR QSPFASSSEH SSENGSLRFS

    TPPGELDGGI SGRSGTGSGG STPHISGPGP GRPSSKEGRR SDTCEYCGKV

    FKNCSNLTVH RRSHTGERPY KCELCNYACA QSSKLTRHMK THGQVGKDVY

    KCEICKMPFS VYSTLEKHMK KWHSDRVLNN DIKTE

  • UCSC uc002sab.4; human. [Q9H165-1];


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