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Catalog
E042922
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species
Mouse
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10 pg/ml
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Intended UseMouse ADP ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Adiponectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Adiponectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Adiponectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Adiponectin can be calculated.
Background: Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene. This gene spans 16 kb, contains 3 exons, and maps to to chromosome 3q27. Adiponectin is a 244-amino-acid-long polypeptide, and it has four distinct regions. It is a hormone secreted by adipocytes (and also from the placenta in pregnancy) that regulates energy homeostasis and glucose and lipid metabolism. Adipocytes also produce and secrete proteins such as leptin, adipsin, various other complement components and tumor necrosis factor, suggesting a possible link to the immune system.
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