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Alternative name
Adiponectin ELISA KIT; 30 kDa adipocyte complement-related protein ELISA KIT; Adipocyte complement-related 30 kDa protein ELISA KIT; ACRP30 ELISA KIT; Adipocyte, C1q and collagen domain-containing protein ELISA KIT; Adipose most abundant gene transcript 1 protein ELISA KIT; apM-1 ELISA KIT; Gelatin-binding proteinADIPOQ ELISA KIT; ACDC ELISA KIT; ACRP30 ELISA KIT; APM1 ELISA KIT; GBP28 ELISA KIT; ACRP30 ELISA KIT; apM-1 ELISA KIT
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Catalog
E042916
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species
Mouse
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GeneADP/Acrp30
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.469ng/ml
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Intended UseMouse ADP/Acrp30 ELISA Kit allows for the in vitro quantitative determination of ADP/Acrp30 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Adiponectin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Adiponectin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Adiponectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Adiponectin can be calculated.
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