Mouse Adipoq ELISA Kit

Alternative name
Mouse 30 kDa adipocyte complement-related protein ELISA Kit;Mouse adipocyte complement-related 30 kDa protein ELISA Kit;Mouse Acrp30 ELISA Kit;Mouse adipocyte, C1q and collagen domain-containing protein ELISA Kit;Mouse adipocyte-specific protein AdipoQ ELISA Kit;Mouse Acdc ELISA Kit;Mouse apM1 ELISA Kit;Mouse 30kDa ELISA Kit;Mouse APN ELISA Kit;Mouse Ad ELISA Kit;Mouse GBP28 ELISA Kit;Mouse adipo ELISA Kit;Mouse adiponectin, C1Q and collagen domain containing ELISA Kit;Mouse adiponectin ELISA Kit;Mouse adipocyte complement related protein ELISA Kit;Mouse adipocyte, C1Q and collagen domain containing ELISA Kit;
Catalog
E042915
species
Mouse
GeneAdipoq
SpecificityNatural and recombinant full length mouse Adiponectin
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Adipoq ELISA Kit allows for the in vitro quantitative determination of Adipoq , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO,E18-N247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Adiponectin amount of sample captured in plate. Background: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
Mouse Adiponectin Protein information
Uniprot ID ADIPO_MOUSE
Uniprot AC Q60994; Q62400; Q6GTX4; Q9DC68;
UniGene Mm.3969;
GeneID 11450
KEGG mmu:11450;
Mouse Adiponectin Protein SEQUENCE
SEQUENCE 247 AA; 26809 MW; 0ECC687D9A8E8123 CRC64;
MLLLQALLFL LILPSHAEDD VTTTEELAPA LVPPPKGTCA GWMAGIPGHP
GHNGTPGRDG RDGTPGEKGE KGDAGLLGPK GETGDVGMTG AEGPRGFPGT
PGRKGEPGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST
GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA
SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
UCSC uc007ytk.1; mouse.;

Mouse Adipoq ELISA Kit

Contact Us