Human ATG2A ELISA Kit

Alternative name
Human KIAA0404 ELISA Kit;Human autophagy related 2A ELISA Kit;Human autophagy-related protein 2 homolog A ELISA Kit;Human ATG2 autophagy related 2 homolog A ELISA Kit;
Catalog
E004257
species
Human
GeneATG2A
Standard Curve
Other Species Mouse Atg2a ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATG2A. No significant cross-reactivity or interference between ATG2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG2A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ATG2A ELISA Kit allows for the in vitro quantitative determination of ATG2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: ATG2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG2A antibody and an ATG2A-HRP conjugate. The assay sample and buffer are incubated together with ATG2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG2A concentration since ATG2A from samples and ATG2A-HRP conjugate compete for the anti-ATG2A antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG2A from the sample, fewer sites are left to bind ATG2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG2A concentration in each sample is interpolated from this standard curve.
Human Autophagy-related protein 2 homolog A Protein information
Uniprot ID ATG2A_HUMAN
Uniprot AC Q2TAZ0; O43154; Q14DM2; Q6ZTV2; Q7Z6K8; Q8IVY5; Q8TAI8; Q96HH7;
UniGene Hs.370671;
GeneID 23130
KEGG hsa:23130;
Human Autophagy-related protein 2 homolog A Protein SEQUENCE
SEQUENCE 1938 AA; 212860 MW; B0072E075E305A33 CRC64;
MSRWLWPWSN CVKERVCRYL LHHYLGHFFQ EHLSLDQLSL DLYKGSVALR
DIHLEIWSVN EVLESMESPL ELVEGFVGSI EVAVPWAALL TDHCTVRVSG
LQLTLQPRRG PAPGAADSQS WASCMTTSLQ LAQECLRDGL PEPSEPPQPL
EGLEMFAQTI ETVLRRIKVT FLDTVVRVEH SPGDGERGVA VEVRVQRLEY
CDEAVRDPSQ APPVDVHQPP AFLHKLLQLA GVRLHYEELP AQEEPPEPPL
QIGSCSGYME LMVKLKQNEA FPGPKLEVAG QLGSLHLLLT PRQLQQLQEL
LSAVSLTDHE GLADKLNKSR PLGAEDLWLI EQDLNQQLQA GAVAEPLSPD
PLTNPLLNLD NTDLFFSMAG LTSSVASALS ELSLSDVDLA SSVRSDMASR
RLSAQAHPAG KMAPNPLLDT MRPDSLLKMT LGGVTLTLLQ TSAPSSGPPD
LATHFFTEFD ATKDGPFGSR DFHHLRPRFQ RACPCSHVRL TGTAVQLSWE
LRTGSRGRRT TSMEVHFGQL EVLECLWPRG TSEPEYTEIL TFPGTLGSQA
SARPCAHLRH TQILRRVPKS RPRRSVACHC HSELALDLAN FQADVELGAL
DRLAALLRLA TVPAEPPAGL LTEPLPAMEQ QTVFRLSAPR ATLRLRFPIA
DLRPEPDPWA GQAVRAEQLR LELSEPQFRS ELSSGPGPPV PTHLELTCSD
LHGIYEDGGK PPVPCLRVSK ALDPKSTGRK YFLPQVVVTV NPQSSSTQWE
VAPEKGEELE LSVESPCELR EPEPSPFSSK RTMYETEEMV IPGDPEEMRT
FQSRTLALSR CSLEVILPSV HIFLPSKEVY ESIYNRINND LLMWEPADLL
PTPDPAAQPS GFPGPSGFWH DSFKMCKSAF KLANCFDLTP DSDSDDEDAH
FFSVGASGGP QAAAPEAPSL HLQSTFSTLV TVLKGRITAL CETKDEGGKR
LEAVHGELVL DMEHGTLFSV SQYCGQPGLG YFCLEAEKAT LYHRAAVDDY
PLPSHLDLPS FAPPAQLAPT IYPSEEGVTE RGASGRKGQG RGPHMLSTAV
RIHLDPHKNV KEFLVTLRLH KATLRHYMAL PEQSWHSQLL EFLDVLDDPV
LGYLPPTVIT ILHTHLFSCS VDYRPLYLPV RVLITAETFT LSSNIIMDTS
TFLLRFILDD SALYLSDKCE VETLDLRRDY VCVLDVDLLE LVIKTWKGST
EGKLSQPLFE LRCSNNVVHV HSCADSCALL VNLLQYVMST GDLHPPPRPP
SPTEIAGQKL SESPASLPSC PPVETALINQ RDLADALLDT ERSLRELAQP
SGGHLPQASP ISVYLFPGER SGAPPPSPPV GGPAGSLGSC SEEKEDEREE
EGDGDTLDSD EFCILDAPGL GIPPRDGEPV VTQLHPGPIV VRDGYFSRPI
GSTDLLRAPA HFPVPSTRVV LREVSLVWHL YGGRDFGPHP GHRARTGLSG
PRSSPSRCSG PNRPQNSWRT QGGSGRQHHV LMEIQLSKVS FQHEVYPAEP
ATGPAAPSQE LEERPLSRQV FIVQELEVRD RLASSQINKF LYLHTSERMP
RRAHSNMLTI KALHVAPTTN LGGPECCLRV SLMPLRLNVD QDALFFLKDF
FTSLVAGINP VVPGETSAEA RPETRAQPSS PLEGQAEGVE TTGSQEAPGG
GHSPSPPDQQ PIYFREFRFT SEVPIWLDYH GKHVTMDQVG TFAGLLIGLA
QLNCSELKLK RLCCRHGLLG VDKVLGYALN EWLQDIRKNQ LPGLLGGVGP
MHSVVQLFQG FRDLLWLPIE QYRKDGRLMR GLQRGAASFG SSTASAALEL
SNRLVQAIQA TAETVYDILS PAAPVSRSLQ DKRSARRLRR GQQPADLREG
VAKAYDTVRE GILDTAQTIC DVASRGHEQK GLTGAVGGVI RQLPPTVVKP
LILATEATSS LLGGMRNQIV PDAHKDHALK WRSDSAQD
UCSC uc001obx.4; human. [Q2TAZ0-1];

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Human ATG2A ELISA Kit

Human KIAA0404 ELISA Kit;Human autophagy related 2A ELISA Kit;Human autophagy-related protein 2 homolog A ELISA Kit;Human ATG2 autophagy related 2 homolog A ELISA Kit;