Human ATG2A ELISA Kit

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  • Alternative name

    Human KIAA0404 ELISA Kit;Human autophagy related 2A ELISA Kit;Human autophagy-related protein 2 homolog A ELISA Kit;Human ATG2 autophagy related 2 homolog A ELISA Kit;

  • Catalog
    E004257
  • species
    Human
  • GeneATG2A
  • Standard CurveHuman ATG2A ELISA Kit
  • Other Species Mouse Atg2a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATG2A. No significant cross-reactivity or interference between ATG2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG2A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATG2A ELISA Kit allows for the in vitro quantitative determination of ATG2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the assay: ATG2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG2A antibody and an ATG2A-HRP conjugate. The assay sample and buffer are incubated together with ATG2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG2A concentration since ATG2A from samples and ATG2A-HRP conjugate compete for the anti-ATG2A antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG2A from the sample, fewer sites are left to bind ATG2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG2A concentration in each sample is interpolated from this standard curve.
  • Human Autophagy-related protein 2 homolog A Protein information
  • Uniprot ID ATG2A_HUMAN
  • Uniprot AC Q2TAZ0; O43154; Q14DM2; Q6ZTV2; Q7Z6K8; Q8IVY5; Q8TAI8; Q96HH7;
  • UniGene Hs.370671;
  • GeneID 23130
  • KEGG hsa:23130;
  • Human Autophagy-related protein 2 homolog A Protein SEQUENCE
  • SEQUENCE 1938 AA; 212860 MW; B0072E075E305A33 CRC64;

    MSRWLWPWSN CVKERVCRYL LHHYLGHFFQ EHLSLDQLSL DLYKGSVALR

    DIHLEIWSVN EVLESMESPL ELVEGFVGSI EVAVPWAALL TDHCTVRVSG

    LQLTLQPRRG PAPGAADSQS WASCMTTSLQ LAQECLRDGL PEPSEPPQPL

    EGLEMFAQTI ETVLRRIKVT FLDTVVRVEH SPGDGERGVA VEVRVQRLEY

    CDEAVRDPSQ APPVDVHQPP AFLHKLLQLA GVRLHYEELP AQEEPPEPPL

    QIGSCSGYME LMVKLKQNEA FPGPKLEVAG QLGSLHLLLT PRQLQQLQEL

    LSAVSLTDHE GLADKLNKSR PLGAEDLWLI EQDLNQQLQA GAVAEPLSPD

    PLTNPLLNLD NTDLFFSMAG LTSSVASALS ELSLSDVDLA SSVRSDMASR

    RLSAQAHPAG KMAPNPLLDT MRPDSLLKMT LGGVTLTLLQ TSAPSSGPPD

    LATHFFTEFD ATKDGPFGSR DFHHLRPRFQ RACPCSHVRL TGTAVQLSWE

    LRTGSRGRRT TSMEVHFGQL EVLECLWPRG TSEPEYTEIL TFPGTLGSQA

    SARPCAHLRH TQILRRVPKS RPRRSVACHC HSELALDLAN FQADVELGAL

    DRLAALLRLA TVPAEPPAGL LTEPLPAMEQ QTVFRLSAPR ATLRLRFPIA

    DLRPEPDPWA GQAVRAEQLR LELSEPQFRS ELSSGPGPPV PTHLELTCSD

    LHGIYEDGGK PPVPCLRVSK ALDPKSTGRK YFLPQVVVTV NPQSSSTQWE

    VAPEKGEELE LSVESPCELR EPEPSPFSSK RTMYETEEMV IPGDPEEMRT

    FQSRTLALSR CSLEVILPSV HIFLPSKEVY ESIYNRINND LLMWEPADLL

    PTPDPAAQPS GFPGPSGFWH DSFKMCKSAF KLANCFDLTP DSDSDDEDAH

    FFSVGASGGP QAAAPEAPSL HLQSTFSTLV TVLKGRITAL CETKDEGGKR

    LEAVHGELVL DMEHGTLFSV SQYCGQPGLG YFCLEAEKAT LYHRAAVDDY

    PLPSHLDLPS FAPPAQLAPT IYPSEEGVTE RGASGRKGQG RGPHMLSTAV

    RIHLDPHKNV KEFLVTLRLH KATLRHYMAL PEQSWHSQLL EFLDVLDDPV

    LGYLPPTVIT ILHTHLFSCS VDYRPLYLPV RVLITAETFT LSSNIIMDTS

    TFLLRFILDD SALYLSDKCE VETLDLRRDY VCVLDVDLLE LVIKTWKGST

    EGKLSQPLFE LRCSNNVVHV HSCADSCALL VNLLQYVMST GDLHPPPRPP

    SPTEIAGQKL SESPASLPSC PPVETALINQ RDLADALLDT ERSLRELAQP

    SGGHLPQASP ISVYLFPGER SGAPPPSPPV GGPAGSLGSC SEEKEDEREE

    EGDGDTLDSD EFCILDAPGL GIPPRDGEPV VTQLHPGPIV VRDGYFSRPI

    GSTDLLRAPA HFPVPSTRVV LREVSLVWHL YGGRDFGPHP GHRARTGLSG

    PRSSPSRCSG PNRPQNSWRT QGGSGRQHHV LMEIQLSKVS FQHEVYPAEP

    ATGPAAPSQE LEERPLSRQV FIVQELEVRD RLASSQINKF LYLHTSERMP

    RRAHSNMLTI KALHVAPTTN LGGPECCLRV SLMPLRLNVD QDALFFLKDF

    FTSLVAGINP VVPGETSAEA RPETRAQPSS PLEGQAEGVE TTGSQEAPGG

    GHSPSPPDQQ PIYFREFRFT SEVPIWLDYH GKHVTMDQVG TFAGLLIGLA

    QLNCSELKLK RLCCRHGLLG VDKVLGYALN EWLQDIRKNQ LPGLLGGVGP

    MHSVVQLFQG FRDLLWLPIE QYRKDGRLMR GLQRGAASFG SSTASAALEL

    SNRLVQAIQA TAETVYDILS PAAPVSRSLQ DKRSARRLRR GQQPADLREG

    VAKAYDTVRE GILDTAQTIC DVASRGHEQK GLTGAVGGVI RQLPPTVVKP

    LILATEATSS LLGGMRNQIV PDAHKDHALK WRSDSAQD

  • UCSC uc001obx.4; human. [Q2TAZ0-1];


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