Mouse Actc1 ELISA Kit

Alternative name
Mouse alpha-cardiac actin ELISA Kit;Mouse Actc ELISA Kit;Mouse Actc-1 ELISA Kit;Mouse actin, alpha, cardiac muscle 1 ELISA Kit;Mouse actin, alpha cardiac muscle 1 ELISA Kit;Mouse actin alpha cardiac ELISA Kit;Mouse alphac-actin ELISA Kit;
Catalog
E042551
species
Mouse
GeneActc1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACTC1. No significant cross-reactivity or interference between ACTC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTC1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Actc1 ELISA Kit allows for the in vitro quantitative determination of Actc1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This ACTC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACTC1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACTC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTC1 antibody and an ACTC1-HRP conjugate. The assay sample and buffer are incubated together with ACTC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTC1 concentration since ACTC1 from samples and ACTC1-HRP conjugate compete for the anti-ACTC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTC1 from the sample, fewer sites are left to bind ACTC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTC1 concentration in each sample is interpolated from this standard curve.
Mouse Actin, alpha cardiac muscle 1 Protein information
Uniprot ID ACTC_MOUSE
Uniprot AC P68033; P04270;
UniGene Mm.686;
GeneID 11464
KEGG mmu:11464;
Mouse Actin, alpha cardiac muscle 1 Protein SEQUENCE
SEQUENCE 377 AA; 42019 MW; E5C10FA19730CAD2 CRC64;
MCDDEETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG
QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP
EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT
TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY
SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI
TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV
LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS
LSTFQQMWIS KQEYDEAGPS IVHRKCF
UCSC uc008lpz.1; mouse.;

Category

Mouse Actc1 ELISA Kit

Mouse alpha-cardiac actin ELISA Kit;Mouse Actc ELISA Kit;Mouse Actc-1 ELISA Kit;Mouse actin, alpha, cardiac muscle 1 ELISA Kit;Mouse actin, alpha cardiac muscle 1 ELISA Kit;Mouse actin alpha cardiac ELISA Kit;Mouse alphac-actin ELISA Kit;