Human ATG5 ELISA Kit

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  • Alternative name

    Human APG5-LIKE ELISA Kit;Human apoptosis-specific protein ELISA Kit;Human APG5L ELISA Kit;Human ASP ELISA Kit;Human APG5 ELISA Kit;Human hAPG5 ELISA Kit;Human autophagy related 5 ELISA Kit;Human autophagy protein 5 ELISA Kit;Human APG5 autophagy 5-like ELISA Kit;Human ATG5 autophagy related 5 homolog ELISA Kit;

  • Catalog
    E004249
  • species
    Human
  • GeneATG5
  • Standard CurveHuman ATG5 ELISA Kit
  • Other Species Mouse Atg5 ELISA KitBovine ATG5 ELISA KitPorcine ATG5 ELISA KitRat Atg5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATG5. No significant cross-reactivity or interference between ATG5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATG5 ELISA Kit allows for the in vitro quantitative determination of ATG5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses||This ATG5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ATG5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG5 antibody and an ATG5-HRP conjugate. The assay sample and buffer are incubated together with ATG5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG5 concentration since ATG5 from samples and ATG5-HRP conjugate compete for the anti-ATG5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG5 from the sample, fewer sites are left to bind ATG5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG5 concentration in each sample is interpolated from this standard curve.
  • Human Autophagy protein 5 Protein information
  • Uniprot ID ATG5_HUMAN
  • Uniprot AC Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7;
  • UniGene Hs.486063;
  • GeneID 9474
  • KEGG hsa:9474;
  • Human Autophagy protein 5 Protein SEQUENCE
  • SEQUENCE 275 AA; 32447 MW; C33A1E0B3C1DBE5C CRC64;

    MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD

    KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI

    TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH

    KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ

    KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP

    LQWLSEHLSY PDNFLHISII PQPTD

  • UCSC uc003prf.4; human. [Q9H1Y0-1];


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