Human ATG5 ELISA Kit

Alternative name
Human APG5-LIKE ELISA Kit;Human apoptosis-specific protein ELISA Kit;Human APG5L ELISA Kit;Human ASP ELISA Kit;Human APG5 ELISA Kit;Human hAPG5 ELISA Kit;Human autophagy related 5 ELISA Kit;Human autophagy protein 5 ELISA Kit;Human APG5 autophagy 5-like ELISA Kit;Human ATG5 autophagy related 5 homolog ELISA Kit;
Catalog
E004249
species
Human
GeneATG5
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATG5. No significant cross-reactivity or interference between ATG5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATG5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ATG5 ELISA Kit allows for the in vitro quantitative determination of ATG5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses||This ATG5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ATG5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG5 antibody and an ATG5-HRP conjugate. The assay sample and buffer are incubated together with ATG5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG5 concentration since ATG5 from samples and ATG5-HRP conjugate compete for the anti-ATG5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG5 from the sample, fewer sites are left to bind ATG5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG5 concentration in each sample is interpolated from this standard curve.
Human Autophagy protein 5 Protein information
Uniprot ID ATG5_HUMAN
Uniprot AC Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7;
UniGene Hs.486063;
GeneID 9474
KEGG hsa:9474;
Human Autophagy protein 5 Protein SEQUENCE
SEQUENCE 275 AA; 32447 MW; C33A1E0B3C1DBE5C CRC64;
MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD
KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI
TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH
KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ
KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP
LQWLSEHLSY PDNFLHISII PQPTD
UCSC uc003prf.4; human. [Q9H1Y0-1];

Human ATG5 ELISA Kit

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