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  • Alternative name

    Tumor necrosis factor ligand superfamily member 13 ELISA KIT; A proliferation-inducing ligand ELISA KIT; APRIL ELISA KIT; TNF- and APOL-related leukocyte expressed ligand 2 ELISA KIT; TALL-2 ELISA KIT; TNF-related death ligand 1 ELISA KIT; TRDL-1 ELISA KIT; CD_antigen: CD256TNFSF13 ELISA KIT; APRIL ELISA KIT; TALL2 ELISA KIT; ZTNF2 ELISA KIT; UNQ383/PRO715 ELISA KIT; APRIL ELISA KIT; TALL-2 ELISA KIT; TRDL-1 ELISA KIT

  • Catalog
  • species
  • GeneAPRIL
  • Standard CurveMouse APRIL ELISA Kit
  • Other Species Human APRIL ELISA KitHuman TNFSF13/APRIL ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of APIL. No significant cross-reactivity or interference between APIL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between APIL and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1ng/mL
  • Intended UseMouse APRIL ELISA Kit allows for the in vitro quantitative determination of APRIL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Intended Uses: This APIL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse APIL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: APIL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APIL antibody and an APIL-HRP conjugate. The assay sample and buffer are incubated together with APIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APIL concentration since APIL from samples and APIL-HRP conjugate compete for the anti-APIL antibody binding site. Since the number of sites is limited, as more sites are occupied by APIL from the sample, fewer sites are left to bind APIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APIL concentration in each sample is interpolated from this standard curve.

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