Human ATXN1 ELISA Kit

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  • Alternative name

    Human spinocerebellar ataxia type 1 protein ELISA Kit;Human ATX1 ELISA Kit;Human SCA1 ELISA Kit;Human D6S504E ELISA Kit;Human ataxin 1 ELISA Kit;Human ataxin-1 ELISA Kit;Human alternative ataxin1 ELISA Kit;

  • Catalog
    E004228
  • species
    Human
  • GeneATXN1
  • Other Species Mouse Atxn1 ELISA KitRat Atxn1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ATXN1. No significant cross-reactivity or interference between Human ATXN1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ATXN1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseHuman ATXN1 ELISA Kit allows for the in vitro quantitative determination of ATXN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATXN1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATXN1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ATXN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATXN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ATXN1. You can calculate the concentration of Human ATXN1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Ataxin-1 Protein information
  • Uniprot ID ATX1_HUMAN
  • Uniprot AC P54253; Q17S02; Q9UJG2; Q9Y4J1;
  • UniGene Hs.434961;
  • GeneID 6310
  • KEGG hsa:6310;
  • Human Ataxin-1 Protein SEQUENCE
  • SEQUENCE 815 AA; 86923 MW; 657876F8FD19ECB2 CRC64;

    MKSNQERSNE CLPPKKREIP ATSRSSEEKA PTLPSDNHRV EGTAWLPGNP

    GGRGHGGGRH GPAGTSVELG LQQGIGLHKA LSTGLDYSPP SAPRSVPVAT

    TLPAAYATPQ PGTPVSPVQY AHLPHTFQFI GSSQYSGTYA SFIPSQLIPP

    TANPVTSAVA SAAGATTPSQ RSQLEAYSTL LANMGSLSQT PGHKAEQQQQ

    QQQQQQQQHQ HQQQQQQQQQ QQQQQHLSRA PGLITPGSPP PAQQNQYVHI

    SSSPQNTGRT ASPPAIPVHL HPHQTMIPHT LTLGPPSQVV MQYADSGSHF

    VPREATKKAE SSRLQQAIQA KEVLNGEMEK SRRYGAPSSA DLGLGKAGGK

    SVPHPYESRH VVVHPSPSDY SSRDPSGVRA SVMVLPNSNT PAADLEVQQA

    THREASPSTL NDKSGLHLGK PGHRSYALSP HTVIQTTHSA SEPLPVGLPA

    TAFYAGTQPP VIGYLSGQQQ AITYAGSLPQ HLVIPGTQPL LIPVGSTDME

    ASGAAPAIVT SSPQFAAVPH TFVTTALPKS ENFNPEALVT QAAYPAMVQA

    QIHLPVVQSV ASPAAAPPTL PPYFMKGSII QLANGELKKV EDLKTEDFIQ

    SAEISNDLKI DSSTVERIED SHSPGVAVIQ FAVGEHRAQV SVEVLVEYPF

    FVFGQGWSSC CPERTSQLFD LPCSKLSVGD VCISLTLKNL KNGSVKKGQP

    VDPASVLLKH SKADGLAGSR HRYAEQENGI NQGSAQMLSE NGELKFPEKM

    GLPAAPFLTK IEPSKPAATR KRRWSAPESR KLEKSEDEPP LTLPKPSLIP

    QEVKICIEGR SNVGK

  • UCSC uc003nbt.4; human. [P54253-1];


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