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Human PLA2G6 ELISA Kit

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  • Alternative name

    Human CaI-PLA2 ELISA Kit;Human Group VI phospholipase A2 ELISA Kit;Human GVI PLA2 ELISA Kit;Human intracellular membrane-associated calcium-independent phospholipase A2 beta ELISA Kit;Human iPLA2-beta ELISA Kit;Human patatin-like phospholipase domain-containing protein 9 ELISA Kit;Human PNPLA9 ELISA Kit;Human PLPLA9 ELISA Kit;Human GVI ELISA Kit;Human INAD1 ELISA Kit;Human IPLA2-VIA ELISA Kit;Human NBIA2 ELISA Kit;Human NBIA2A ELISA Kit;Human NBIA2B ELISA Kit;Human PARK14 ELISA Kit;Human PLA2 ELISA Kit;Human iPLA2 ELISA Kit;Human iPLA2beta ELISA Kit;Human phospholipase A2 group VI ELISA Kit;Human 85/88 kDa calcium-independent phospholipase A2 ELISA Kit;Human 85 kDa calcium-independent phospholipase A2 ELISA Kit;Human neurodegeneration with brain iron accumulation 2 ELISA Kit;Human phospholipase A2, group VI (cytosolic, calcium-independent) ELISA Kit;
  • Catalog
    E042225
  • species
    Human
  • GenePLA2G6
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman PLA2G6 ELISA Kit allows for the in vitro quantitative determination of PLA2G6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human iPLA2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human iPLA2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human iPLA2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human iPLA2 can be calculated.
  • Human 85/88 kDa calcium-independent phospholipase A2 Protein information
  • Uniprot ID PLPL9_HUMAN
  • Uniprot AC O60733; A8K597; B0QYE8; O75645; Q8N452; Q9UG29; Q9UIT0; Q9Y671;
  • UniGene Hs.170479;
  • GeneID 8398
  • KEGG hsa:8398;
  • Human 85/88 kDa calcium-independent phospholipase A2 Protein SEQUENCE

  • SEQUENCE 806 AA; 89903 MW; 8E55CD4EB9ACAD8B CRC64;

    MQFFGRLVNT FSGVTNLFSN PFRVKEVAVA DYTSSDRVRE EGQLILFQNT

    PNRTWDCVLV NPRNSQSGFR LFQLELEADA LVNFHQYSSQ LLPFYESSPQ

    VLHTEVLQHL TDLIRNHPSW SVAHLAVELG IRECFHHSRI ISCANCAENE

    EGCTPLHLAC RKGDGEILVE LVQYCHTQMD VTDYKGETVF HYAVQGDNSQ

    VLQLLGRNAV AGLNQVNNQG LTPLHLACQL GKQEMVRVLL LCNARCNIMG

    PNGYPIHSAM KFSQKGCAEM IISMDSSQIH SKDPRYGASP LHWAKNAEMA

    RMLLKRGCNV NSTSSAGNTA LHVAVMRNRF DCAIVLLTHG ANADARGEHG

    NTPLHLAMSK DNVEMIKALI VFGAEVDTPN DFGETPTFLA SKIGRLVTRK

    AILTLLRTVG AEYCFPPIHG VPAEQGSAAP HHPFSLERAQ PPPISLNNLE

    LQDLMHISRA RKPAFILGSM RDEKRTHDHL LCLDGGGVKG LIIIQLLIAI

    EKASGVATKD LFDWVAGTST GGILALAILH SKSMAYMRGM YFRMKDEVFR

    GSRPYESGPL EEFLKREFGE HTKMTDVRKP KVMLTGTLSD RQPAELHLFR

    NYDAPETVRE PRFNQNVNLR PPAQPSDQLV WRAARSSGAA PTYFRPNGRF

    LDGGLLANNP TLDAMTEIHE YNQDLIRKGQ ANKVKKLSIV VSLGTGRSPQ

    VPVTCVDVFR PSNPWELAKT VFGAKELGKM VVDCCTDPDG RAVDRARAWC

    EMVGIQYFRL NPQLGTDIML DEVSDTVLVN ALWETEVYIY EHREEFQKLI

    QLLLSP

  • UCSC uc003auy.2; human. [O60733-1];



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