Human 8-iso-PGF2a ELISA Kit

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  • Catalog
    E042219
  • species
    Human
  • Gene8-iso-PGF2a
  • Other Species Mouse 8-iso-PGF2a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human 8-iso-PGF2a. No significant cross-reactivity or interference between human 8-iso-PGF2a and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human 8-iso-PGF2a is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined
  • Intended UseHuman 8-iso-PGF2a ELISA Kit allows for the in vitro quantitative determination of 8-iso-PGF2a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 8-iso-PGF2a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 8-iso-PGF2a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 8-iso-PGF2a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-iso-PGF2a bound in the initial step. The color development is stopped and the intensity of the color is measured.


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