Human DHCR7 ELISA Kit

Alternative name
Human 7-DHC reductase ELISA Kit;Human putative sterol reductase SR-2 ELISA Kit;Human Sterol Delta(7)-reductase ELISA Kit;Human D7SR ELISA Kit;Human SLOS ELISA Kit;Human 7-dehydrocholesterol reductase ELISA Kit;Human delta-7-dehydrocholesterol reductase ELISA Kit;Human sterol delta-7-reductase ELISA Kit;
Catalog
E042201
species
Human
GeneDHCR7
SpecificityThis assay has high sensitivity and excellent specificity for detection of human DHCR7. No significant cross-reactivity or interference between human DHCR7 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human DHCR7 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman DHCR7 ELISA Kit allows for the in vitro quantitative determination of DHCR7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DHCR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHCR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 7-dehydrocholesterol reductase Protein information
Uniprot ID DHCR7_HUMAN
Uniprot AC GGGHLLPY FYIIYMAILL THRCLRDEHR CASKYGRDWE RYTAAVPYRL LPGIF
UniGene Hs.503134;
GeneID 1717
KEGG hsa:1717;
Human 7-dehydrocholesterol reductase Protein SEQUENCE
SEQUENCE 475 AA; 54489 MW; 7D726443834C4EEB CRC64;
MAAKSQPNIP KAKSLDGVTN DRTASQGQWG RAWEVDWFSL ASVIFLLLFA
PFIVYYFIMA CDQYSCALTG PVVDIVTGHA RLSDIWAKTP PITRKAAQLY
TLWVTFQVLL YTSLPDFCHK FLPGYVGGIQ EGAVTPAGVV NKYQINGLQA
WLLTHLLWFA NAHLLSWFSP TIIFDNWIPL LWCANILGYA VSTFAMVKGY
FFPTSARDCK FTGNFFYNYM MGIEFNPRIG KWFDFKLFFN GRPGIVAWTL
INLSFAAKQR ELHSHVTNAM VLVNVLQAIY VIDFFWNETW YLKTIDICHD
HFGWYLGWGD CVWLPYLYTL QGLYLVYHPV QLSTPHAVGV LLLGLVGYYI
FRVANHQKDL FRRTDGRCLI WGRKPKVIEC SYTSADGQRH HSKLLVSGFW
GVARHFNYVG DLMGSLAYCL ACGGGHLLPY FYIIYMAILL THRCLRDEHR
CASKYGRDWE RYTAAVPYRL LPGIF
UCSC uc001oqk.4; human.;

Category

Human DHCR7 ELISA Kit