Human RPL6 ELISA Kit

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  • Alternative name

    Human neoplasm-related protein C140 ELISA Kit;Human tax-responsive enhancer element-binding protein 107 ELISA Kit;Human TAXREB107 ELISA Kit;Human TXREB1 ELISA Kit;Human L6 ELISA Kit;Human SHUJUN-2 ELISA Kit;Human ribosomal protein L6 ELISA Kit;Human 60S ribosomal protein L6 ELISA Kit;Human DNA-binding protein TAXREB107 ELISA Kit;

  • Catalog
    E042192
  • species
    Human
  • GeneRPL6
  • Standard CurveHuman RPL6 ELISA Kit
  • Other Species Mouse Rpl6 ELISA KitBovine RPL6 ELISA KitPorcine RPL6 ELISA KitRat Rpl6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RPL6. No significant cross-reactivity or interference between RPL6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPL6 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RPL6 ELISA Kit allows for the in vitro quantitative determination of RPL6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This RPL6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPL6. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RPL6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPL6 antibody and an RPL6-HRP conjugate. The assay sample and buffer are incubated together with RPL6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPL6 concentration since RPL6 from samples and RPL6-HRP conjugate compete for the anti-RPL6 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPL6 from the sample, fewer sites are left to bind RPL6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPL6 concentration in each sample is interpolated from this standard curve.
  • Human 60S ribosomal protein L6 Protein information
  • Uniprot ID RL6_HUMAN
  • Uniprot AC Q02878; Q2M3Q3; Q8WW97;
  • UniGene Hs.528668; Hs.546283; Hs.744897;
  • GeneID 6128
  • KEGG hsa:6128;
  • Human 60S ribosomal protein L6 Protein SEQUENCE
  • SEQUENCE 288 AA; 32728 MW; D24A6168C7D0ECBF CRC64;

    MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL

    VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVLA TVTKPVGGDK

    NGGTRVVKLR KMPRYYPTED VPRKLLSHGK KPFSQHVRKL RASITPGTIL

    IILTGRHRGK RVVFLKQLAS GLLLVTGPLV LNRVPLRRTH QKFVIATSTK

    IDISNVKIPK HLTDAYFKKK KLRKPRHQEG EIFDTEKEKY EITEQRKIDQ

    KAVDSQILPK IKAIPQLQGY LRSVFALTNG IYPHKLVF

  • UCSC uc001ttu.4; human.;


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