Human HSPD1 ELISA Kit

Alternative name
Human 60 kDa chaperonin ELISA Kit;Human chaperonin 60 ELISA Kit;Human CPN60 ELISA Kit;Human Heat shock protein 60 ELISA Kit;Human HSP-60 ELISA Kit;Human HSP60 ELISA Kit;Human HuCHA60 ELISA Kit;Human mitochondrial matrix protein P1 ELISA Kit;Human P60 lymphocyte protein ELISA Kit;Human GROEL ELISA Kit;Human HLD4 ELISA Kit;Human HSP65 ELISA Kit;Human SPG13 ELISA Kit;Human heat shock protein family D (Hsp60) member 1 ELISA Kit;Human 60 kDa heat shock protein, mitochondrial ELISA Kit;Human heat shock 60kDa protein 1 (chaperonin) ELISA Kit;Human heat shock protein 65 ELISA Kit;Human short heat shock protein 60 Hsp60s1 ELISA Kit;
Catalog
E042147
species
Human
GeneHSPD1
Standard Curve
Other Species Human HSP-60/HSPD1 ELISA Kit Human HSPD1/HSP-60 ELISA Kit Mouse Hspd1 ELISA Kit Bovine HSPD1 ELISA Kit Canine HSPD1 ELISA Kit Chicken HSPD1 ELISA Kit Hamster HSPD1 ELISA Kit Rat Hspd1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of HSPD1. No significant cross-reactivity or interference between HSPD1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSPD1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman HSPD1 ELISA Kit allows for the in vitro quantitative determination of HSPD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: HSPD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSPD1 antibody and an HSPD1-HRP conjugate. The assay sample and buffer are incubated together with HSPD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPD1 concentration since HSPD1 from samples and HSPD1-HRP conjugate compete for the anti-HSPD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPD1 from the sample, fewer sites are left to bind HSPD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPD1 concentration in each sample is interpolated from this standard curve.
Human 60 kDa heat shock protein, mitochondrial Protein information
Uniprot ID CH60_HUMAN
Uniprot AC P10809; B2R5M6; B7Z712; Q38L19; Q9UCR6;
UniGene Hs.595053; Hs.727543;
GeneID 3329
KEGG hsa:3329;
Human 60 kDa heat shock protein, mitochondrial Protein SEQUENCE
SEQUENCE 573 AA; 61055 MW; E51E1BAD9615899C CRC64;
MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALM LQGVDLLADA
VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD
VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA
VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT
VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK
ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV
KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV
TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG
VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA
LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE
VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP
KEEKDPGMGA MGGMGGGMGG GMF
UCSC uc002uui.4; human. [P10809-1];

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Human HSPD1 ELISA Kit