Human HTR4 ELISA Kit

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  • Alternative name

    Human 5-HT-4 ELISA Kit;Human 5-HT4 ELISA Kit;Human Serotonin receptor 4 ELISA Kit;Human 5-HT4R ELISA Kit;Human 5-hydroxytryptamine receptor 4 ELISA Kit;Human 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled ELISA Kit;Human cardiac 5-HT4 receptor ELISA Kit;

  • Catalog
    E042087
  • species
    Human
  • GeneHTR4
  • Standard CurveHuman HTR4 ELISA Kit
  • Other Species Human 5-HTR4 ELISA KitMouse Htr4 ELISA KitGuinea Pig HTR4 ELISA KitPorcine HTR4 ELISA KitRat Htr4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine Receptor 4 (HTR4). No significant cross-reactivity or interference between 5-Hydroxytryptamine Receptor 4 (HTR4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman HTR4 ELISA Kit allows for the in vitro quantitative determination of HTR4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 4 (HTR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 4 (HTR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 4 (HTR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 4 (HTR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 5-hydroxytryptamine receptor 4 Protein information
  • Uniprot ID 5HT4R_HUMAN
  • Uniprot AC Q9NY73; Q9UBM6; Q9UBT4; Q9UE22; Q9UE23; Q9UQR6;
  • UniGene Hs.483773;
  • GeneID 3360
  • KEGG hsa:3360;
  • Human 5-hydroxytryptamine receptor 4 Protein SEQUENCE
  • SEQUENCE 388 AA; 43761 MW; 7FCFEC60E7BDF560 CRC64;

    MDKLDANVSS EEGFGSVEKV VLLTFLSTVI LMAILGNLLV MVAVCWDRQL

    RKIKTNYFIV SLAFADLLVS VLVMPFGAIE LVQDIWIYGE VFCLVRTSLD

    VLLTTASIFH LCCISLDRYY AICCQPLVYR NKMTPLRIAL MLGGCWVIPT

    FISFLPIMQG WNNIGIIDLI EKRKFNQNSN STYCVFMVNK PYAITCSVVA

    FYIPFLLMVL AYYRIYVTAK EHAHQIQMLQ RAGASSESRP QSADQHSTHR

    MRTETKAAKT LCIIMGCFCL CWAPFFVTNI VDPFIDYTVP GQVWTAFLWL

    GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYRRPSIL GQTVPCSTTT

    INGSTHVLRD AVECGGQWES QCHPPATSPL VAAQPSDT

  • UCSC uc003lpn.5; human. [Q13639-1];


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