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Human HTR2A ELISA Kit

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  • Alternative name

    Human 5-HT-2 ELISA Kit;Human 5-HT-2A ELISA Kit;Human Serotonin receptor 2A ELISA Kit;Human HTR2 ELISA Kit;Human 5-HT2A ELISA Kit;Human 5-hydroxytryptamine receptor 2A ELISA Kit;Human 5-HT2 receptor ELISA Kit;Human 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled ELISA Kit;Human serotonin 5-HT-2A receptor ELISA Kit;
  • Catalog
    E042083
  • species
    Human
  • GeneHTR2A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine Receptor 2A (HTR2A). No significant cross-reactivity or interference between 5-Hydroxytryptamine Receptor 2A (HTR2A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman HTR2A ELISA Kit allows for the in vitro quantitative determination of HTR2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 2A (HTR2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 2A (HTR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 2A (HTR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 2A (HTR2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 5-hydroxytryptamine receptor 2A Protein information
  • Uniprot ID 5HT2A_HUMAN
  • Uniprot AC P28223; B2RAC5; B4DZ79; F5GWE8; Q5T8C0;
  • UniGene Hs.72630;
  • GeneID 3356
  • KEGG hsa:3356;
  • Human 5-hydroxytryptamine receptor 2A Protein SEQUENCE

  • SEQUENCE 471 AA; 52603 MW; EF8AAC0BC5379DA2 CRC64;

    MDILCEENTS LSSTTNSLMQ LNDDTRLYSN DFNSGEANTS DAFNWTVDSE

    NRTNLSCEGC LSPSCLSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS

    LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV

    WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHSRFNSRT KAFLKIIAVW

    TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM

    VITYFLTIKS LQKEATLCVS DLGTRAKLAS FSFLPQSSLS SEKLFQRSIH

    REPGSYTGRR TMQSISNEQK ACKVLGIVFF LFVVMWCPFF ITNIMAVICK

    ESCNEDVIGA LLNVFVWIGY LSSAVNPLVY TLFNKTYRSA FSRYIQCQYK

    ENKKPLQLIL VNTIPALAYK SSQLQMGQKK NSKQDAKTTD NDCSMVALGK

    QHSEEASKDN SDGVNEKVSC V

  • UCSC uc001vbr.5; human. [P28223-1];



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