Human HTR1B ELISA Kit

Alternative name
Human 5-HT-1B ELISA Kit;Human 5-HT1B ELISA Kit;Human S12 ELISA Kit;Human serotonin 1D beta receptor ELISA Kit;Human 5-HT-1D-beta ELISA Kit;Human serotonin receptor 1B ELISA Kit;Human HTR1DB ELISA Kit;Human 5-HT1DB ELISA Kit;Human HTR1D2 ELISA Kit;Human 5-hydroxytryptamine receptor 1B ELISA Kit;Human 5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled ELISA Kit;
Catalog
E042079
species
Human
GeneHTR1B
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman HTR1B ELISA Kit allows for the in vitro quantitative determination of HTR1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HTR1B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HTR1B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HTR1B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HTR1B can be calculated.
Human 5-hydroxytryptamine receptor 1B Protein information
Uniprot ID 5HT1B_HUMAN
Uniprot AC P28222; Q4VAY7;
UniGene Hs.123016;
GeneID 3351
KEGG hsa:3351;
Human 5-hydroxytryptamine receptor 1B Protein SEQUENCE
SEQUENCE 390 AA; 43568 MW; CD874DC7EB44CF12 CRC64;
MEEPGAQCAP PPPAGSETWV PQANLSSAPS QNCSAKDYIY QDSISLPWKV
LLVMLLALIT LATTLSNAFV IATVYRTRKL HTPANYLIAS LAVTDLLVSI
LVMPISTMYT VTGRWTLGQV VCDFWLSSDI TCCTASILHL CVIALDRYWA
ITDAVEYSAK RTPKRAAVMI ALVWVFSISI SLPPFFWRQA KAEEEVSECV
VNTDHILYTV YSTVGAFYFP TLLLIALYGR IYVEARSRIL KQTPNRTGKR
LTRAQLITDS PGSTSSVTSI NSRVPDVPSE SGSPVYVNQV KVRVSDALLE
KKKLMAARER KATKTLGIIL GAFIVCWLPF FIISLVMPIC KDACWFHLAI
FDFFTWLGYL NSLINPIIYT MSNEDFKQAF HKLIRFKCTS

Category

Human HTR1B ELISA Kit