Human ATP1B4 ELISA Kit

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  • Alternative name

    Human X,K-ATPase subunit beta-m ELISA Kit;Human x/potassium-transporting ATPase subunit beta-m ELISA Kit;Human ATPase Na+/K+ transporting family member beta 4 ELISA Kit;Human protein ATP1B4 ELISA Kit;Human ATPase, Na+/K+ transporting, beta 4 polypeptide ELISA Kit;Human BetaM ELISA Kit;Human Na,K-ATPase beta m-subunit ELISA Kit;Human X,K-ATPase beta-m subunit ELISA Kit;

  • Catalog
    E004201
  • species
    Human
  • GeneATP1B4
  • Other Species Mouse Atp1b4 ELISA KitBovine ATP1B4 ELISA KitChicken ATP1B4 ELISA KitPorcine ATP1B4 ELISA KitRat Atp1b4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.106ng/mL.
  • Intended UseHuman ATP1B4 ELISA Kit allows for the in vitro quantitative determination of ATP1B4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein ATP1B4 Protein information
  • Uniprot ID AT1B4_HUMAN
  • Uniprot AC Q9UN42; Q17RR0; Q9UN41;
  • UniGene Hs.292599;
  • GeneID 23439
  • KEGG hsa:23439;
  • Human Protein ATP1B4 Protein SEQUENCE
  • SEQUENCE 357 AA; 41598 MW; 94926EB1BB637ACA CRC64;

    MRRQLRSRRA PSFPYSYRYR LDDPDEANQN YLADEEEEAE EEARVTVVPK

    SEEEEEEEEK EEEEEEEKEE EEGQGQPTGN AWWQKLQIMS EYLWDPERRM

    FLARTGQSWS LILLIYFFFY ASLAAVITLC MYTLFLTISP YIPTFTERVK

    PPGVMIRPFA HSLNFNFNVS EPDTWQHYVI SLNGFLQGYN DSLQEEMNVD

    CPPGQYFIQD GNEDEDKKAC QFKRSFLKNC SGLEDPTFGY STGQPCILLK

    MNRIVGFRPE LGDPVKVSCK VQRGDENDIR SISYYPESAS FDLRYYPYYG

    KLTHVNYTSP LVAMHFTDVV KNQAVPVQCQ LKGKGVINDV INDRFVGRVI

    FTLNIET

  • UCSC uc004esq.4; human. [Q9UN42-1];



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