Human 4-HNE ELISA Kit

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  • Catalog
    E041990
  • species
    Human
  • Gene4-HNE
  • Other Species Human 4 HNE ELISA KitMouse 4-HNE ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Human 4-HNE. No significant cross-reactivity or interference between Human 4-HNE and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.375ng/mL
  • Intended UseHuman 4-HNE ELISA Kit allows for the in vitro quantitative determination of 4-HNE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human 4-HNE concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 4-HNE. During the reaction, 4-HNE in the sample or standard competes with a fixed amount of 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 4-HNE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.


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