Human ABAT ELISA Kit

Alternative name
Human (S)-3-amino-2-methylpropionate transaminase ELISA Kit;Human GABA aminotransferase ELISA Kit;Human GABA-AT ELISA Kit;Human gamma-amino-N-butyrate transaminase ELISA Kit;Human GABA transaminase ELISA Kit;Human GABA-T ELISA Kit;Human L-AIBAT ELISA Kit;Human GABAT ELISA Kit;Human NPD009 ELISA Kit;Human 4-aminobutyrate aminotransferase ELISA Kit;Human 4-aminobutyrate aminotransferase, mitochondrial ELISA Kit;Human 4-aminobutyrate transaminase ELISA Kit;Human GABA transferase ELISA Kit;
Catalog
E041983
species
Human
GeneABAT
Standard Curve
Other Species Mouse Abat ELISA Kit Bovine ABAT ELISA Kit Porcine ABAT ELISA Kit Rat Abat ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ABAT. No significant cross-reactivity or interference between ABAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ABAT and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ABAT ELISA Kit allows for the in vitro quantitative determination of ABAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: ABAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABAT antibody and an ABAT-HRP conjugate. The assay sample and buffer are incubated together with ABAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABAT concentration since ABAT from samples and ABAT-HRP conjugate compete for the anti-ABAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ABAT from the sample, fewer sites are left to bind ABAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABAT concentration in each sample is interpolated from this standard curve.
Human 4-aminobutyrate aminotransferase, mitochondrial Protein information
Uniprot ID GABT_HUMAN
Uniprot AC P80404; A8K386; Q16260; Q8N5W2; Q96BG2; Q99800;
UniGene Hs.336768;
GeneID 18
KEGG hsa:18;
Human 4-aminobutyrate aminotransferase, mitochondrial Protein SEQUENCE
SEQUENCE 500 AA; 56439 MW; F990B0B6B77BD3F5 CRC64;
MASMLLAQRL ACSFQHSYRL LVPGSRHISQ AAAKVDVEFD YDGPLMKTEV
PGPRSQELMK QLNIIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI
SSVPIGYSHP ALLKLIQQPQ NASMFVNRPA LGILPPENFV EKLRQSLLSV
APKGMSQLIT MACGSCSNEN ALKTIFMWYR SKERGQRGFS QEELETCMIN
QAPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR
LKYPLEEFVK ENQQEEARCL EEVEDLIVKY RKKKKTVAGI IVEPIQSEGG
DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA
DVMTFSKKMM TGGFFHKEEF RPNAPYRIFN TWLGDPSKNL LLAEVINIIK
REDLLNNAAH AGKALLTGLL DLQARYPQFI SRVRGRGTFC SFDTPDDSIR
NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLN IFSDILADFK

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Human ABAT ELISA Kit