SpecificityThis assay has high sensitivity and excellent specificity for detection of MRPL52. No significant cross-reactivity or interference between MRPL52 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPL52 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman MRPL52 ELISA Kit allows for the in vitro quantitative determination of MRPL52 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: MRPL52 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRPL52 antibody and an MRPL52-HRP conjugate. The assay sample and buffer are incubated together with MRPL52-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPL52 concentration since MRPL52 from samples and MRPL52-HRP conjugate compete for the anti-MRPL52 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPL52 from the sample, fewer sites are left to bind MRPL52-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPL52 concentration in each sample is interpolated from this standard curve.
Human 39S ribosomal protein L52, mitochondrial Protein information
Q86TS9; A6NMQ8; A8MXK5; A8MYI6; G3XCN9; Q6NVH8;
Human 39S ribosomal protein L52, mitochondrial Protein SEQUENCE