Human MRPL52 ELISA Kit

Alternative name
Human L52mt ELISA Kit;Human MRP-L52 ELISA Kit;Human mitochondrial ribosomal protein L52 ELISA Kit;Human 39S ribosomal protein L52, mitochondrial ELISA Kit;
Catalog
E041977
species
Human
GeneMRPL52
SpecificityThis assay has high sensitivity and excellent specificity for detection of MRPL52. No significant cross-reactivity or interference between MRPL52 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPL52 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman MRPL52 ELISA Kit allows for the in vitro quantitative determination of MRPL52 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: MRPL52 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRPL52 antibody and an MRPL52-HRP conjugate. The assay sample and buffer are incubated together with MRPL52-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPL52 concentration since MRPL52 from samples and MRPL52-HRP conjugate compete for the anti-MRPL52 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPL52 from the sample, fewer sites are left to bind MRPL52-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPL52 concentration in each sample is interpolated from this standard curve.
Human 39S ribosomal protein L52, mitochondrial Protein information
Uniprot ID RM52_HUMAN
Uniprot AC Q86TS9; A6NMQ8; A8MXK5; A8MYI6; G3XCN9; Q6NVH8;
UniGene Hs.355935;
GeneID 122704
KEGG hsa:122704;
Human 39S ribosomal protein L52, mitochondrial Protein SEQUENCE
SEQUENCE 123 AA; 13664 MW; A67803930E27E4EE CRC64;
MAALGTVLFT GVRRLHCSVA AWAGGQWRLQ QGLAANPSGY GPLTELPDWS
YADGRPAPPM KGQLRRKAER ETFARRVVLL SQEMDAGLQA WQLRQQKLQE
EQRKQENALK PKGASLKSPL PSQ
UCSC uc001wgw.4; human. [Q86TS9-1];

Human MRPL52 ELISA Kit

Human L52mt ELISA Kit;Human MRP-L52 ELISA Kit;Human mitochondrial ribosomal protein L52 ELISA Kit;Human 39S ribosomal protein L52, mitochondrial ELISA Kit;

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