Human BDH1 ELISA Kit

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  • Alternative name

    Human 3-hydroxybutyrate dehydrogenase ELISA Kit;Human BDH ELISA Kit;Human short chain dehydrogenase/reductase family 9C member 1 ELISA Kit;Human SDR9C1 ELISA Kit;Human 3-hydroxybutyrate dehydrogenase 1 ELISA Kit;Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial ELISA Kit;Human (R)-3-hydroxybutyrate dehydrogenase ELISA Kit;Human 3-hydroxybutyrate dehydrogenase (heart, mitochondrial) ELISA Kit;Human 3-hydroxybutyrate dehydrogenase, type 1 ELISA Kit;

  • Catalog
    E041927
  • species
    Human
  • GeneBDH1
  • Standard CurveHuman BDH1 ELISA Kit
  • Other Species Mouse Bdh1 ELISA KitBovine BDH1 ELISA KitChicken BDH1 ELISA KitRat Bdh1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 3-Hydroxybutyrate Dehydrogenase 1 (BDH1). No significant cross-reactivity or interference between 3-Hydroxybutyrate Dehydrogenase 1 (BDH1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman BDH1 ELISA Kit allows for the in vitro quantitative determination of BDH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxybutyrate Dehydrogenase 1 (BDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxybutyrate Dehydrogenase 1 (BDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxybutyrate Dehydrogenase 1 (BDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Hydroxybutyrate Dehydrogenase 1 (BDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial Protein information
  • Uniprot ID BDH_HUMAN
  • Uniprot AC Q02338; D3DXC0; Q96ET1; Q9BRZ4;
  • UniGene Hs.274539;
  • GeneID 622
  • KEGG hsa:622;
  • Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial Protein SEQUENCE
  • SEQUENCE 343 AA; 38157 MW; B8AA1148111ACA8F CRC64;

    MLATRLSRPL SRLPGKTLSA CDRENGARRP LLLGSTSFIP IGRRTYASAA

    EPVGSKAVLV TGCDSGFGFS LAKHLHSKGF LVFAGCLMKD KGHDGVKELD

    SLNSDRLRTV QLNVCSSEEV EKVVEIVRSS LKDPEKGMWG LVNNAGISTF

    GEVEFTSLET YKQVAEVNLW GTVRMTKSFL PLIRRAKGRV VNISSMLGRM

    ANPARSPYCI TKFGVEAFSD CLRYEMYPLG VKVSVVEPGN FIAATSLYSP

    ESIQAIAKKM WEELPEVVRK DYGKKYFDEK IAKMETYCSS GSTDTSPVID

    AVTHALTATT PYTRYHPMDY YWWLRMQIMT HLPGAISDMI YIR

  • UCSC uc003fxr.3; human.;


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