Human KDSR ELISA Kit

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  • Alternative name

    Human KDS reductase ELISA Kit;Human 3-dehydrosphinganine reductase ELISA Kit;Human follicular variant translocation protein 1 ELISA Kit;Human FVT-1 ELISA Kit;Human short chain dehydrogenase/reductase family 35C member 1 ELISA Kit;Human FVT1 ELISA Kit;Human SDR35C1 ELISA Kit;Human DHSR ELISA Kit;Human 3-ketodihydrosphingosine reductase ELISA Kit;Human follicular lymphoma variant translocation 1 ELISA Kit;

  • Catalog
    E041907
  • species
    Human
  • GeneKDSR
  • Standard CurveHuman KDSR ELISA Kit
  • Other Species Mouse Kdsr ELISA KitBovine KDSR ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of KDSR. No significant cross-reactivity or interference between KDSR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KDSR and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman KDSR ELISA Kit allows for the in vitro quantitative determination of KDSR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: KDSR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KDSR antibody and an KDSR-HRP conjugate. The assay sample and buffer are incubated together with KDSR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KDSR concentration since KDSR from samples and KDSR-HRP conjugate compete for the anti-KDSR antibody binding site. Since the number of sites is limited, as more sites are occupied by KDSR from the sample, fewer sites are left to bind KDSR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KDSR concentration in each sample is interpolated from this standard curve.
  • Human 3-ketodihydrosphingosine reductase Protein information
  • Uniprot ID KDSR_HUMAN
  • Uniprot AC Q06136; B2R5Y1; B4DMX0;
  • UniGene Hs.74050;
  • GeneID 2531
  • KEGG hsa:2531;
  • Human 3-ketodihydrosphingosine reductase Protein SEQUENCE
  • SEQUENCE 332 AA; 36187 MW; CB4BA5D020858F0C CRC64;

    MLLLAAAFLV AFVLLLYMVS PLISPKPLAL PGAHVVVTGG SSGIGKCIAI

    ECYKQGAFIT LVARNEDKLL QAKKEIEMHS INDKQVVLCI SVDVSQDYNQ

    VENVIKQAQE KLGPVDMLVN CAGMAVSGKF EDLEVSTFER LMSINYLGSV

    YPSRAVITTM KERRVGRIVF VSSQAGQLGL FGFTAYSASK FAIRGLAEAL

    QMEVKPYNVY ITVAYPPDTD TPGFAEENRT KPLETRLISE TTSVCKPEQV

    AKQIVKDAIQ GNFNSSLGSD GYMLSALTCG MAPVTSITEG LQQVVTMGLF

    RTIALFYLGS FDSIVRRCMM QREKSENADK TA

  • UCSC uc010dpw.4; human. [Q06136-1];


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