Human 27HC ELISA Kit

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  • Catalog
  • species
  • Gene27HC
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human 27-hydroxycholesterol. No significant cross-reactivity or interference between Human 27-hydroxycholesterol and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.82 pg/mL (mean of 6 independent assays)
  • Intended UseHuman 27HC ELISA Kit allows for the in vitro quantitative determination of 27HC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: For the quantitative detection of Human 27-hydroxycholesterol concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 27-hydroxycholesterol. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)- conjugated 27-hydroxycholesterol and incubated. The competitive inhibition reaction is launched between with HRP labeled 27-hydroxycholesterol and unlabeled 27-hydroxycholesterol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of 27-hydroxycholesterol in the sample. The color development is stopped and the intensity of the color is measured.

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