Human 25OHVD ELISA Kit

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  • Catalog
    E041829
  • species
    Human
  • Gene25OHVD
  • Standard CurveHuman 25OHVD ELISA Kit
  • Other Species Human 25OHVD ELISA KitMouse 25OHVD ELISA KitBovine 25OHVD ELISA KitCanine 25OHVD ELISA KitChicken 25OHVD ELISA KitGeneral 25OHVD ELISA KitPorcine 25OHVD ELISA KitRabbit 25OHVD ELISA KitRat 25OHVD ELISA KitCamel 25OHVD ELISA KitGoat 25OHVD ELISA KitGuinea Pig 25OHVD ELISA KitHamster 25OHVD ELISA KitHorse 25OHVD ELISA KitMonkey 25OHVD ELISA KitSheep 25OHVD ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 25-OHVD. No significant cross-reactivity or interference between 25-OHVD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 25-OHVD and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman 25OHVD ELISA Kit allows for the in vitro quantitative determination of 25OHVD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyMetabolism
  • Product Description
    specifical
    Principle of the assay: 25-OHVD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-25-OHVD antibody and an 25-OHVD-HRP conjugate. The assay sample and buffer are incubated together with 25-OHVD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 25-OHVD concentration since 25-OHVD from samples and 25-OHVD-HRP conjugate compete for the anti-25-OHVD antibody binding site. Since the number of sites is limited, as more sites are occupied by 25-OHVD from the sample, fewer sites are left to bind 25-OHVD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 25-OHVD concentration in each sample is interpolated from this standard curve.



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