SpecificityThis assay has high sensitivity and excellent specificity for detection of 25-OHVD. No significant cross-reactivity or interference between 25-OHVD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 25-OHVD and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman 25OHVD ELISA Kit allows for the in vitro quantitative determination of 25OHVD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: 25-OHVD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-25-OHVD antibody and an 25-OHVD-HRP conjugate. The assay sample and buffer are incubated together with 25-OHVD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 25-OHVD concentration since 25-OHVD from samples and 25-OHVD-HRP conjugate compete for the anti-25-OHVD antibody binding site. Since the number of sites is limited, as more sites are occupied by 25-OHVD from the sample, fewer sites are left to bind 25-OHVD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 25-OHVD concentration in each sample is interpolated from this standard curve.