Human 25OHVD ELISA Kit

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  • Alternative name

    25-OH Vitamin D ELISA Kit,25OHVD ELISA Kit,Calcifediol ELISA Kit,Calcidiol ELISA Kit

  • Catalog
    E041823
  • species
    Human
  • Gene25OHVD
  • Standard CurveHuman 25OHVD ELISA Kit
  • Other Species Human 25OHVD ELISA KitMouse 25OHVD ELISA KitBovine 25OHVD ELISA KitCanine 25OHVD ELISA KitChicken 25OHVD ELISA KitGeneral 25OHVD ELISA KitPorcine 25OHVD ELISA KitRabbit 25OHVD ELISA KitRat 25OHVD ELISA KitCamel 25OHVD ELISA KitGoat 25OHVD ELISA KitGuinea Pig 25OHVD ELISA KitHamster 25OHVD ELISA KitHorse 25OHVD ELISA KitMonkey 25OHVD ELISA KitSheep 25OHVD ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.78nmol/L
  • Intended UseHuman 25OHVD ELISA Kit allows for the in vitro quantitative determination of 25OHVD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical
    Human 25OHVD ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 25OHVD, During the reaction,25OHVD in the sample or standard competes with a fixed amount of biotin-labeled 25OHVD for sites on a pre-coated Monoclonal antibody specific to 25OHVD. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 25OHVD in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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