Human LOX 12 ELISA Kit

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  • Alternative name

    Arachidonate 15-lipoxygenase ELISA KIT; 12/15-lipoxygenase ELISA KIT; Arachidonate 12-lipoxygenase, leukocyte-type (EC: ELISA KIT; 12-LOX ELISA KIT; Arachidonate omega-6 lipoxygenaseALOX15 ELISA KIT; LOG15 ELISA KIT; 15-LOX ELISA KIT; 15-LOX-1 ELISA KIT; 12-LOX ELISA KIT

  • Catalog
  • species
  • GeneLOX 12
  • Other Species Mouse LOX-12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LOX12. No significant cross-reactivity or interference between LOX12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LOX12 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman LOX 12 ELISA Kit allows for the in vitro quantitative determination of LOX 12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This LOX12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LOX12. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LOX12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LOX12. Standards or samples are then added to the microtiter plate wells and LOX12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LOX12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LOX12 are added to each well to "sandwich" the LOX12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LOX12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX12 concentration in each sample is interpolated from this standard curve.

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