Human 11,12-DHETs ELISA Kit

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  • Catalog
  • species
  • Gene11,12-DHETs
  • SpecificityThis kit recognizes natural and recombinant Human 11,12-DHETs. No significant cross-reactivity or interference between Human 11,12-DHETs and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human 11,12-DHETs is 0.938ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman 11,12-DHETs ELISA Kit allows for the in vitro quantitative determination of 11,12-DHETs , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 11,12-DHETs. During the reaction, 11,12-DHETs in the sample or standard competes with a fixed amount of 11,12-DHETs on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 11,12-DHETs. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 11,12-DHETs in the samples is then determined by comparing the OD of the samples to the standard curve.

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