SpecificityThis assay has high sensitivity and excellent specificity for detection of 11 Deoxycortisol. No significant cross-reactivity or interference between 11 Deoxycortisol and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 11 Deoxycortisol and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman 11 DH TXB2 ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses||This 11 Deoxycortisol ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 11 Deoxycortisol. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: 11 Deoxycortisol ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-11 Deoxycortisol antibody and an 11 Deoxycortisol-HRP conjugate. The assay sample and buffer are incubated together with 11 Deoxycortisol-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 11 Deoxycortisol concentration since 11 Deoxycortisol from samples and 11 Deoxycortisol-HRP conjugate compete for the anti-11 Deoxycortisol antibody binding site. Since the number of sites is limited, as more sites are occupied by 11 Deoxycortisol from the sample, fewer sites are left to bind 11 Deoxycortisol-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 11 Deoxycortisol concentration in each sample is interpolated from this standard curve.