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Human ATAD5 ELISA Kit

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  • Alternative name

    Human chromosome fragility-associated gene 1 protein ELISA Kit;Human C17orf41 ELISA Kit;Human FRAG1 ELISA Kit;Human ELG1 ELISA Kit;Human ATPase family, AAA domain containing 5 ELISA Kit;Human ATPase family AAA domain-containing protein 5 ELISA Kit;Human chromosome fragility associated gene 1 ELISA Kit;Human enhanced level of genomic instability 1 homolog ELISA Kit;
  • Catalog
    E004141
  • species
    Human
  • GeneATAD5
  • Standard CurveHuman ATAD5 ELISA Kit
  • Other Species Mouse Atad5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATAD5. No significant cross-reactivity or interference between ATAD5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATAD5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1ng/mL.
  • Intended UseHuman ATAD5 ELISA Kit allows for the in vitro quantitative determination of ATAD5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This ATAD5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATAD5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ATAD5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATAD5 antibody and an ATAD5-HRP conjugate. The assay sample and buffer are incubated together with ATAD5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATAD5 concentration since ATAD5 from samples and ATAD5-HRP conjugate compete for the anti-ATAD5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATAD5 from the sample, fewer sites are left to bind ATAD5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATAD5 concentration in each sample is interpolated from this standard curve.
  • Human ATPase family AAA domain-containing protein 5 Protein information
  • Uniprot ID ATAD5_HUMAN
  • Uniprot AC Q96QE3; Q05DH0; Q69YR6; Q9H9I1;
  • UniGene Hs.528902;
  • GeneID 79915
  • KEGG hsa:79915;
  • Human ATPase family AAA domain-containing protein 5 Protein SEQUENCE

  • SEQUENCE 1844 AA; 207570 MW; A09A3C76CFC77BD1 CRC64;

    MVGVLAMAAA AAPPPVKDCE IEPCKKRKKD DDTSTCKTIT KYLSPLGKTR

    DRVFAPPKPS NILDYFRKTS PTNEKTQLGK ECKIKSPESV PVDSNKDCTT

    PLEMFSNVEF KKKRKRVNLS HQLNNIKTEN EAPIEISSDD SKEDYSLNND

    FVESSTSVLR YKKQVEVLAE NIQDTKSQPN TMTSLQNSKK VNPKQGTTKN

    DFKKLRKRKC RDVVDLSESL PLAEELNLLK KDGKDTKQME NTTSHANSRD

    NVTEAAQLND SIITVSYEEF LKSHKENKVE EIPDSTMSIC VPSETVDEIV

    KSGYISESEN SEISQQVRFK TVTVLAQVHP IPPKKTGKIP RIFLKQKQFE

    MENSLSDPEN EQTVQKRKSN VVIQEEELEL AVLEAGSSEA VKPKCTLEER

    QQFMKAFRQP ASDALKNGVK KSSDKQKDLN EKCLYEVGRD DNSKKIMENS

    GIQMVSKNGN LQLHTDKGSF LKEKNKKLKK KNKKTLDTGA IPGKNREGNT

    QKKETTFFLK EKQYQNRMSL RQRKTEFFKS STLFNNESLV YEDIANDDLL

    KVSSLCNNNK LSRKTSIPVK DIKLTQSKAE SEASLLNVST PKSTRRSGRI

    SSTPTTETIR GIDSDDVQDN SQLKASTQKA ANLSEKHSLY TAELITVPFD

    SESPIRMKFT RISTPKKSKK KSNKRSEKSE ATDGGFTSQI RKASNTSKNI

    SKAKQLIEKA KALHISRSKV TEEIAIPLRR SSRHQTLPER KKLSETEDSV

    IIIDSSPTAL KHPEKNQKKL QCLNDVLGKK LNTSTKNVPG KMKVAPLFLV

    RKAQKAADPV PSFDESSQDT SEKSQDCDVQ CKAKRDFLMS GLPDLLKRQI

    AKKAAALDVY NAVSTSFQRV VHVQQKDDGC CLWHLKPPSC PLLTKFKELN

    TKVIDLSKCG IALGEFSTLN SKLKSGNSAA VFMRTRKEFT EEVRNLLLEE

    IRWSNPEFSL KKYFPLLLKK QIEHQVLSSE CHSKQELEAD VSHKETKRKL

    VEAENSKSKR KKPNEYSKNL EKTNRKSEEL SKRNNSSGIK LDSSKDSGTE

    DMLWTEKYQP QTASELIGNE LAIKKLHSWL KDWKRRAELE ERQNLKGKRD

    EKHEDFSGGI DFKGSSDDEE ESRLCNTVLI TGPTGVGKTA AVYACAQELG

    FKIFEVNASS QRSGRQILSQ LKEATQSHQV DKQGVNSQKP CFFNSYYIGK

    SPKKISSPKK VVTSPRKVPP PSPKSSGPKR ALPPKTLANY FKVSPKPKNN

    EEIGMLLENN KGIKNSFEQK QITQTKSTNA TNSNVKDVGA EEPSRKNATS

    LILFEEVDVI FDEDAGFLNA IKTFMATTKR PVILTTSDPT FSLMFDGCFE

    EIKFSTPSLL NVASYLQMIC LTENFRTDVK DFVTLLTANT CDIRKSILYL

    QFWIRSGGGV LEERPLTLYR GNSRNVQLVC SEHGLDNKIY PKNTKKKRVD

    LPKCDSGCAE TLFGLKNIFS PSEDLFSFLK HKITMKEEWH KFIQLLTEFQ

    MRNVDFLYSN LEFILPLPVD TIPETKNFCG PSVTVDASAA TKSMNCLARK

    HSEREQPLKK SQKKKQKKTL VILDDSDLFD TDLDFPDQSI SLSSVSSSSN

    AEESKTGDEE SKARDKGNNP ETKKSIPCPP KTTAGKKCSA LVSHCLNSLS

    EFMDNMSFLD ALLTDVREQN KYGRNDFSWT NGKVTSGLCD EFSLESNDGW

    TSQSSGELKA AAEALSFTKC SSAISKALET LNSCKKLGRD PTNDLTFYVS

    QKRNNVYFSQ SAANLDNAWK RISVIKSVFS SRSLLYVGNR QASIIEYLPT

    LRNICKTEKL KEQGKSKRRF LHYFEGIHLD IPKETVNTLA ADFP

  • UCSC uc002hfs.2; human. [Q96QE3-1];


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