Human ZBP1 ELISA Kit

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  • Alternative name

    Human tumor stroma and activated macrophage protein DLM-1 ELISA Kit;Human C20orf183 ELISA Kit;Human DLM1 ELISA Kit;Human DAI ELISA Kit;Human DLM-1 ELISA Kit;Human Z-DNA binding protein 1 ELISA Kit;Human Z-DNA-binding protein 1 ELISA Kit;Human DNA-dependent activator of IRFs ELISA Kit;

  • Catalog
    E040809
  • species
    Human
  • GeneZBP1
  • Standard CurveHuman ZBP1 ELISA Kit
  • Other Species Mouse Zbp1 ELISA KitRat Zbp1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ZBP1 ELISA Kit allows for the in vitro quantitative determination of ZBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Intended Uses: This ZBP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZBP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ZBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZBP1 antibody and an ZBP1-HRP conjugate. The assay sample and buffer are incubated together with ZBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZBP1 concentration since ZBP1 from samples and ZBP1-HRP conjugate compete for the anti-ZBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZBP1 from the sample, fewer sites are left to bind ZBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZBP1 concentration in each sample is interpolated from this standard curve.
  • Human Z-DNA-binding protein 1 Protein information
  • Uniprot ID ZBP1_HUMAN
  • Uniprot AC Q9H171; A2A2F7; B3KVA1; F5GYT1; Q5JY39; Q9BYW4;
  • UniGene Hs.302123;
  • GeneID 81030
  • KEGG hsa:81030;
  • Human Z-DNA-binding protein 1 Protein SEQUENCE
  • SEQUENCE 429 AA; 46343 MW; 2C9B195112822991 CRC64;

    MAQAPADPGR EGHLEQRILQ VLTEAGSPVK LAQLVKECQA PKRELNQVLY

    RMKKELKVSL TSPATWCLGG TDPEGEGPAE LALSSPAERP QQHAATIPET

    PGPQFSQQRE EDIYRFLKDN GPQRALVIAQ ALGMRTAKDV NRDLYRMKSR

    HLLDMDEQSK AWTIYRPEDS GRRAKSASII YQHNPINMIC QNGPNSWISI

    ANSEAIQIGH GNIITRQTVS REDGSAGPRH LPSMAPGDSS TWGTLVDPWG

    PQDIHMEQSI LRRVQLGHSN EMRLHGVPSE GPAHIPPGSP PVSATAAGPE

    ASFEARIPSP GTHPEGEAAQ RIHMKSCFLE DATIGNSNKM SISPGVAGPG

    GVAGSGEGEP GEDAGRRPAD TQSRSHFPRD IGQPITPSHS KLTPKLETMT

    LGNRSHKAAE GSHYVDEASH EGSWWGGGI

  • UCSC uc002xyo.4; human. [Q9H171-1];


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