Human XIRP1 ELISA Kit

Alternative name
Human cardiomyopathy-associated protein 1 ELISA Kit;Human CMYA1 ELISA Kit;Human Xin ELISA Kit;Human xin actin binding repeat containing 1 ELISA Kit;Human xin actin-binding repeat-containing protein 1 ELISA Kit;Human cardiomyopathy associated 1 ELISA Kit;
Catalog
E040760
species
Human
GeneXIRP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of XIRP1. No significant cross-reactivity or interference between XIRP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between XIRP1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman XIRP1 ELISA Kit allows for the in vitro quantitative determination of XIRP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This XIRP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human XIRP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||XIRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XIRP1 antibody and an XIRP1-HRP conjugate. The assay sample and buffer are incubated together with XIRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XIRP1 concentration since XIRP1 from samples and XIRP1-HRP conjugate compete for the anti-XIRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by XIRP1 from the sample, fewer sites are left to bind XIRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XIRP1 concentration in each sample is interpolated from this standard curve.
Human Xin actin-binding repeat-containing protein 1 Protein information
Uniprot ID XIRP1_HUMAN
Uniprot AC Q8N904; Q8TCG7;
UniGene Hs.447868; Hs.737550;
GeneID 165904
KEGG hsa:165904;
Human Xin actin-binding repeat-containing protein 1 Protein SEQUENCE
SEQUENCE 1843 AA; 198561 MW; 41A5CC13FE1955CB CRC64;
MADTQTQVAP TPTMRMATAE DLPLPPPPAL EDLPLPPPKE SFSKFHQQRQ
ASELRRLYRH IHPELRKNLA EAVAEDLAEV LGSEEPTEGD VQCMRWIFEN
WRLDAIGEHE RPAAKEPVLC GDVQATSRKF EEGSFANSTD QEPTRPQPGG
GDVRAARWLF ETKPLDELTG QAKELEATVR EPAASGDVQG TRMLFETRPL
DRLGSRPSLQ EQSPLELRSE IQELKGDVKK TVKLFQTEPL CAIQDAEGAI
HEVKAACREE IQSNAVRSAR WLFETRPLDA INQDPSQVRV IRGISLEEGA
RPDVSATRWI FETQPLDAIR EILVDEKDFQ PSPDLIPPGP DVQQQQHLFE
TRALDTLKGD EEAGAEAPPK EEVVPGDVRS TLWLFETKPL DAFRDKVQVG
HLQRVDPQDG EGHLSSDSSS ALPFSQSAPQ RDELKGDVKT FKNLFETLPL
DSIGQGEVLA HGSPSREEGT DSAGQAQGIG SPVYAMQDSK GRLHALTSVS
REQIVGGDVQ GYRWMFETQP LDQLGRSPST IDVVRGITRQ EVVAGDVGTA
RWLFETQPLE MIHQREQQER QKEEGKSQGD PQPEAPPKGD VQTIRWLFET
CPMSELAEKQ GSEVTDPTAK AEAQSCTWMF KPQPVDRPVG SREQHLQVSQ
VPAGERQTDR HVFETEPLQA SGRPCGRRPV RYCSRVEIPS GQVSRQKEVF
QALEAGKKEE QEPRVIAGSI PAGSVHKFTW LFENCPMGSL AAESIQGGNL
LEEQPMSPSG NRMQESQETA AEGTLRTLHA TPGILHHGGI LMEARGPGEL
CLAKYVLSGT GQGHPYIRKE ELVSGELPRI ICQVLRRPDV DQQGLLVQED
PTGQLQLKPL RLPTPGSSGN IEDMDPELQQ LLACGLGTSV ARTGLVMQET
EQGLVALTAY SLQPRLTSKA SERSSVQLLA SCIDKGDLSG LHSLRWEPPA
DPSPVPASEG AQSLHPTESI IHVPPLDPSM GMGHLRASGA TPCPPQAIGK
AVPLAGEAAA PAQLQNTEKQ EDSHSGQKGM AVLGKSEGAT TTPPGPGAPD
LLAAMQSLRM ATAEAQSLHQ QVLNKHKQGP TPTATSNPIQ DGLRKAGATQ
SNIRPGGGSD PRIPAAPRKV SREEQALPRG LPGGWVTIQD GIYTAHPVRT
FDPPGGVQLS QREPQSRHRE TALSVQAPRP LQGGPGQSTG PGREEPGGCT
QMAWGPPGKA MAEVCPGGLQ AAETTLKTAP LGRHILASGP QAAGASPHPH
NAFVPPPPTL PAAVTGPDFP AGAHRAEDSI QQASEPLKDP LLHSHSSPAG
QRTPGGSQTK TPKLDPTMPP KKKPQLPPKP AHLTQSHPPQ RLPKPLPLSP
SFSSEVGQRE HQRGERDTAI PQPAKVPTTV DQGHIPLARC PSGHSQPSLQ
HGLSTTAPRP TKNQATGSNA QSSEPPKLNA LNHDPTSPQW GPGPSGEQPM
EGSHQGAPES PDSLQRNQKE LQGLLNQVQA LEKEAASSVD VQALRRLFEA
VPQLGGAAPQ APAAHQKPEA SVEQAFGELT RVSTEVAQLK EQTLARLLDI
EEAVHKALSS MSSLQPEASA RGHFQGPPKD HSAHKISVTV SSSARPSGSG
QEVGGQTAVK NQAKVECHTE AQSQVKIRNH TEARGHTAST APSTRRQETS
REYLCPPRVL PSSRDSPSSP TFISIQSATR KPLETPSFKG NPDVSVKSTQ
LAQDIGQALL HQKGVQDKTG KKDITQCSVQ PEPAPPSASP LPRGWQKSVL
ELQTGPGSSQ HYGAMRTVTE QYEEVDQFGN TVLMSSTTVT EQAEPPRNPG
SHLGLHASPL LRQFLHSPAG FSSDLTEAET VQVSCSYSQP AAQ
UCSC uc003cji.4; human. [Q702N8-1];

Category

Human XIRP1 ELISA Kit

Human cardiomyopathy-associated protein 1 ELISA Kit;Human CMYA1 ELISA Kit;Human Xin ELISA Kit;Human xin actin binding repeat containing 1 ELISA Kit;Human xin actin-binding repeat-containing protein 1 ELISA Kit;Human cardiomyopathy associated 1 ELISA Kit;