Human XIRP1 ELISA Kit

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  • Alternative name

    Human cardiomyopathy-associated protein 1 ELISA Kit;Human CMYA1 ELISA Kit;Human Xin ELISA Kit;Human xin actin binding repeat containing 1 ELISA Kit;Human xin actin-binding repeat-containing protein 1 ELISA Kit;Human cardiomyopathy associated 1 ELISA Kit;

  • Catalog
    E040760
  • species
    Human
  • GeneXIRP1
  • Standard CurveHuman XIRP1 ELISA Kit
  • Other Species Mouse Xirp1 ELISA KitChicken Xirp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of XIRP1. No significant cross-reactivity or interference between XIRP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between XIRP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman XIRP1 ELISA Kit allows for the in vitro quantitative determination of XIRP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This XIRP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human XIRP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||XIRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XIRP1 antibody and an XIRP1-HRP conjugate. The assay sample and buffer are incubated together with XIRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XIRP1 concentration since XIRP1 from samples and XIRP1-HRP conjugate compete for the anti-XIRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by XIRP1 from the sample, fewer sites are left to bind XIRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XIRP1 concentration in each sample is interpolated from this standard curve.
  • Human Xin actin-binding repeat-containing protein 1 Protein information
  • Uniprot ID XIRP1_HUMAN
  • Uniprot AC Q8N904; Q8TCG7;
  • UniGene Hs.447868; Hs.737550;
  • GeneID 165904
  • KEGG hsa:165904;
  • Human Xin actin-binding repeat-containing protein 1 Protein SEQUENCE
  • SEQUENCE 1843 AA; 198561 MW; 41A5CC13FE1955CB CRC64;

    MADTQTQVAP TPTMRMATAE DLPLPPPPAL EDLPLPPPKE SFSKFHQQRQ

    ASELRRLYRH IHPELRKNLA EAVAEDLAEV LGSEEPTEGD VQCMRWIFEN

    WRLDAIGEHE RPAAKEPVLC GDVQATSRKF EEGSFANSTD QEPTRPQPGG

    GDVRAARWLF ETKPLDELTG QAKELEATVR EPAASGDVQG TRMLFETRPL

    DRLGSRPSLQ EQSPLELRSE IQELKGDVKK TVKLFQTEPL CAIQDAEGAI

    HEVKAACREE IQSNAVRSAR WLFETRPLDA INQDPSQVRV IRGISLEEGA

    RPDVSATRWI FETQPLDAIR EILVDEKDFQ PSPDLIPPGP DVQQQQHLFE

    TRALDTLKGD EEAGAEAPPK EEVVPGDVRS TLWLFETKPL DAFRDKVQVG

    HLQRVDPQDG EGHLSSDSSS ALPFSQSAPQ RDELKGDVKT FKNLFETLPL

    DSIGQGEVLA HGSPSREEGT DSAGQAQGIG SPVYAMQDSK GRLHALTSVS

    REQIVGGDVQ GYRWMFETQP LDQLGRSPST IDVVRGITRQ EVVAGDVGTA

    RWLFETQPLE MIHQREQQER QKEEGKSQGD PQPEAPPKGD VQTIRWLFET

    CPMSELAEKQ GSEVTDPTAK AEAQSCTWMF KPQPVDRPVG SREQHLQVSQ

    VPAGERQTDR HVFETEPLQA SGRPCGRRPV RYCSRVEIPS GQVSRQKEVF

    QALEAGKKEE QEPRVIAGSI PAGSVHKFTW LFENCPMGSL AAESIQGGNL

    LEEQPMSPSG NRMQESQETA AEGTLRTLHA TPGILHHGGI LMEARGPGEL

    CLAKYVLSGT GQGHPYIRKE ELVSGELPRI ICQVLRRPDV DQQGLLVQED

    PTGQLQLKPL RLPTPGSSGN IEDMDPELQQ LLACGLGTSV ARTGLVMQET

    EQGLVALTAY SLQPRLTSKA SERSSVQLLA SCIDKGDLSG LHSLRWEPPA

    DPSPVPASEG AQSLHPTESI IHVPPLDPSM GMGHLRASGA TPCPPQAIGK

    AVPLAGEAAA PAQLQNTEKQ EDSHSGQKGM AVLGKSEGAT TTPPGPGAPD

    LLAAMQSLRM ATAEAQSLHQ QVLNKHKQGP TPTATSNPIQ DGLRKAGATQ

    SNIRPGGGSD PRIPAAPRKV SREEQALPRG LPGGWVTIQD GIYTAHPVRT

    FDPPGGVQLS QREPQSRHRE TALSVQAPRP LQGGPGQSTG PGREEPGGCT

    QMAWGPPGKA MAEVCPGGLQ AAETTLKTAP LGRHILASGP QAAGASPHPH

    NAFVPPPPTL PAAVTGPDFP AGAHRAEDSI QQASEPLKDP LLHSHSSPAG

    QRTPGGSQTK TPKLDPTMPP KKKPQLPPKP AHLTQSHPPQ RLPKPLPLSP

    SFSSEVGQRE HQRGERDTAI PQPAKVPTTV DQGHIPLARC PSGHSQPSLQ

    HGLSTTAPRP TKNQATGSNA QSSEPPKLNA LNHDPTSPQW GPGPSGEQPM

    EGSHQGAPES PDSLQRNQKE LQGLLNQVQA LEKEAASSVD VQALRRLFEA

    VPQLGGAAPQ APAAHQKPEA SVEQAFGELT RVSTEVAQLK EQTLARLLDI

    EEAVHKALSS MSSLQPEASA RGHFQGPPKD HSAHKISVTV SSSARPSGSG

    QEVGGQTAVK NQAKVECHTE AQSQVKIRNH TEARGHTAST APSTRRQETS

    REYLCPPRVL PSSRDSPSSP TFISIQSATR KPLETPSFKG NPDVSVKSTQ

    LAQDIGQALL HQKGVQDKTG KKDITQCSVQ PEPAPPSASP LPRGWQKSVL

    ELQTGPGSSQ HYGAMRTVTE QYEEVDQFGN TVLMSSTTVT EQAEPPRNPG

    SHLGLHASPL LRQFLHSPAG FSSDLTEAET VQVSCSYSQP AAQ

  • UCSC uc003cji.4; human. [Q702N8-1];



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