Human XPC ELISA Kit

Alternative name
Human Xeroderma pigmentosum group C-complementing protein ELISA Kit;Human p125 ELISA Kit;Human XPCC ELISA Kit;Human RAD4 ELISA Kit;Human XP3 ELISA Kit;Human XPC complex subunit, DNA damage recognition and repair factor ELISA Kit;Human DNA repair protein complementing XP-C cells ELISA Kit;Human mutant xeroderma pigmentosum group C ELISA Kit;Human xeroderma pigmentosum, complementation group C ELISA Kit;
Catalog
E040750
species
Human
GeneXPC
SpecificityThis assay has high sensitivity and excellent specificity for detection of XPC. No significant cross-reactivity or interference between XPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between XPC and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman XPC ELISA Kit allows for the in vitro quantitative determination of XPC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: XPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XPC antibody and an XPC-HRP conjugate. The assay sample and buffer are incubated together with XPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XPC concentration since XPC from samples and XPC-HRP conjugate compete for the anti-XPC antibody binding site. Since the number of sites is limited, as more sites are occupied by XPC from the sample, fewer sites are left to bind XPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XPC concentration in each sample is interpolated from this standard curve.
Human DNA repair protein complementing XP-C cells Protein information
Uniprot ID XPC_HUMAN
Uniprot AC Q01831; B4DIP3; E9PB96; E9PH69; Q53GT7; Q96AX0;
UniGene Hs.475538; Hs.739296;
GeneID 7508
KEGG hsa:7508;
Human DNA repair protein complementing XP-C cells Protein SEQUENCE
SEQUENCE 940 AA; 105953 MW; 2F8C80D43FAA1256 CRC64;
MARKRAAGGE PRGRELRSQK SKAKSKARRE EEEEDAFEDE KPPKKSLLSK
VSQGKRKRGC SHPGGSADGP AKKKVAKVTV KSENLKVIKD EALSDGDDLR
DFPSDLKKAH HLKRGATMNE DSNEEEEESE NDWEEVEELS EPVLGDVRES
TAFSRSLLPV KPVEIEIETP EQAKTRERSE KIKLEFETYL RRAMKRFNKG
VHEDTHKVHL LCLLANGFYR NNICSQPDLH AIGLSIIPAR FTRVLPRDVD
TYYLSNLVKW FIGTFTVNAE LSASEQDNLQ TTLERRFAIY SARDDEELVH
IFLLILRALQ LLTRLVLSLQ PIPLKSATAK GKKPSKERLT ADPGGSSETS
SQVLENHTKP KTSKGTKQEE TFAKGTCRPS AKGKRNKGGR KKRSKPSSSE
EDEGPGDKQE KATQRRPHGR ERRVASRVSY KEESGSDEAG SGSDFELSSG
EASDPSDEDS EPGPPKQRKA PAPQRTKAGS KSASRTHRGS HRKDPSLPAA
SSSSSSSKRG KKMCSDGEKA EKRSIAGIDQ WLEVFCEQEE KWVCVDCVHG
VVGQPLTCYK YATKPMTYVV GIDSDGWVRD VTQRYDPVWM TVTRKCRVDA
EWWAETLRPY QSPFMDREKK EDLEFQAKHM DQPLPTAIGL YKNHPLYALK
RHLLKYEAIY PETAAILGYC RGEAVYSRDC VHTLHSRDTW LKKARVVRLG
EVPYKMVKGF SNRARKARLA EPQLREENDL GLFGYWQTEE YQPPVAVDGK
VPRNEFGNVY LFLPSMMPIG CVQLNLPNLH RVARKLDIDC VQAITGFDFH
GGYSHPVTDG YIVCEEFKDV LLTAWENEQA VIERKEKEKK EKRALGNWKL
LAKGLLIRER LKRRYGPKSE AAAPHTDAGG GLSSDEEEGT SSQAEAARIL
AASWPQNRED EEKQKLKGGP KKTKREKKAA ASHLFPFEQL
UCSC uc062gzd.1; human. [Q01831-1];

Category

Human XPC ELISA Kit