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Human ATP5B ELISA Kit

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  • Alternative name

    Human ATPMB ELISA Kit;Human ATPSB ELISA Kit;Human HEL-S-271 ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide ELISA Kit;Human ATP synthase subunit beta, mitochondrial ELISA Kit;Human epididymis secretory protein Li 271 ELISA Kit;Human mitochondrial ATP synthase beta subunit ELISA Kit;Human mitochondrial ATP synthetase, beta subunit ELISA Kit;
  • Catalog
    E004073
  • species
    Human
  • GeneATP5B
  • Other Species Mouse Atp5b ELISA KitBovine ATP5B ELISA KitCanine ATP5B ELISA KitChicken ATP5B ELISA KitRat Atp5b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ATP5B. No significant cross-reactivity or interference between human ATP5B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ATP5B is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman ATP5B ELISA Kit allows for the in vitro quantitative determination of ATP5B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATP5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human ATP synthase subunit beta, mitochondrial Protein information
  • Uniprot ID ATPB_HUMAN
  • Uniprot AC P06576; A8K4X0; Q14283;
  • UniGene Hs.406510;
  • GeneID 506
  • KEGG hsa:506;
  • Human ATP synthase subunit beta, mitochondrial Protein SEQUENCE

  • SEQUENCE 529 AA; 56560 MW; 960C616A2252B91A CRC64;

    MLGFVGRVAA APASGALRRL TPSASLPPAQ LLLRAAPTAV HPVRDYAAQT

    SPSPKAGAAT GRIVAVIGAV VDVQFDEGLP PILNALEVQG RETRLVLEVA

    QHLGESTVRT IAMDGTEGLV RGQKVLDSGA PIKIPVGPET LGRIMNVIGE

    PIDERGPIKT KQFAPIHAEA PEFMEMSVEQ EILVTGIKVV DLLAPYAKGG

    KIGLFGGAGV GKTVLIMELI NNVAKAHGGY SVFAGVGERT REGNDLYHEM

    IESGVINLKD ATSKVALVYG QMNEPPGARA RVALTGLTVA EYFRDQEGQD

    VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG TMQERITTTK

    KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDP

    LDSTSRIMDP NIVGSEHYDV ARGVQKILQD YKSLQDIIAI LGMDELSEED

    KLTVSRARKI QRFLSQPFQV AEVFTGHMGK LVPLKETIKG FQQILAGEYD

    HLPEQAFYMV GPIEEAVAKA DKLAEEHSS



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