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Alternative name
Human ATPase subunit F6 ELISA Kit;Human ATP5A ELISA Kit;Human ATPM ELISA Kit;Human ATP5 ELISA Kit;Human CF6 ELISA Kit;Human F6 ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 ELISA Kit;Human ATP synthase-coupling factor 6, mitochondrial ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 ELISA Kit;Human coupling factor 6 ELISA Kit;Human mitochondrial ATP synthase, coupling factor 6 ELISA Kit;Human mitochondrial ATP synthase, subunit F6 ELISA Kit;Human mitochondrial ATPase coupling factor 6 ELISA Kit;Human proliferation-inducing protein 36 ELISA Kit;
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Catalog
E004071
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species
Human
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GeneATP5J
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Other Species
Mouse Atp5j ELISA KitBovine ATP5J ELISA KitMonkey ATP5J ELISA KitPorcine ATP5J ELISA KitRat Atp5j ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human CF6. No significant cross-reactivity or interference between human CF6 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human CF6 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
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Intended UseHuman ATP5J ELISA Kit allows for the in vitro quantitative determination of ATP5J , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CF6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Human ATP synthase-coupling factor 6, mitochondrial Protein information
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Uniprot ID
ATP5J_HUMAN
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Uniprot AC
P18859; J3KQ83;
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UniGene
Hs.246310;
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GeneID
522
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KEGG
hsa:522;
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Human ATP synthase-coupling factor 6, mitochondrial Protein SEQUENCE
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SEQUENCE 108 AA; 12588 MW; EDC1A14F01A10F17 CRC64;
MILQRLFRFS SVIRSAVSVH LRRNIGVTAV AFNKELDPIQ KLFVDKIREY
KSKRQTSGGP
VDASSEYQQE LERELFKLKQ MFGNADMNTF PTFKFEDPKF
EVIEKPQA
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UCSC
uc002ylv.4; human. [P18859-1];
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