SensitivityThe minimum detectable dose of human CF6 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman ATP5J ELISA Kit allows for the in vitro quantitative determination of ATP5J , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CF6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human ATP synthase-coupling factor 6, mitochondrial Protein information
Human ATP synthase-coupling factor 6, mitochondrial Protein SEQUENCE