Human ATP5H ELISA Kit

Alternative name
Human ATPase subunit d ELISA Kit;Human ATPQ ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial Fo complex subunit D ELISA Kit;Human ATP synthase subunit d, mitochondrial ELISA Kit;Human ATP synthase D chain, mitochondrial ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial F1F0, subunit d ELISA Kit;Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d ELISA Kit;Human My032 protein ELISA Kit;
Catalog
E004070
species
Human
GeneATP5H
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ATP5H. No significant cross-reactivity or interference between human ATP5H and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ATP5H is typically less than 9.7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman ATP5H ELISA Kit allows for the in vitro quantitative determination of ATP5H , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATP5H has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP5H present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP5H is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP5H bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human ATP synthase subunit d, mitochondrial Protein information
Uniprot ID ATP5H_HUMAN
Uniprot AC O75947; B2R5L6; Q9H3J4;
UniGene Hs.514465;
GeneID 10476
KEGG hsa:10476;
Human ATP synthase subunit d, mitochondrial Protein SEQUENCE
SEQUENCE 161 AA; 18491 MW; E93020ADA1BA1694 CRC64;
MAGRKLALKT IDWVAFAEII PQNQKAIASS LKSWNETLTS RLAALPENPP
AIDWAYYKAN VAKAGLVDDF EKKFNALKVP VPEDKYTAQV DAEEKEDVKS
CAEWVSLSKA RIVEYEKEME KMKNLIPFDQ MTIEDLNEAF PETKLDKKKY
PYWPHQPIEN L
UCSC uc002jmn.2; human. [O75947-1];

Human ATP5H ELISA Kit

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