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Alternative name
A disintegrin and metalloproteinase with thrombospondin motifs 13 ELISA KIT; von Willebrand factor-cleaving protease ELISA KIT; vWF-CP ELISA KIT; vWF-cleaving proteaseADAMTS13 ELISA KIT; C9orf8 ELISA KIT; ADAM-TS 13 ELISA KIT; ADAM-TS13 ELISA KIT; ADAMTS-13 ELISA KIT; vWF-CP ELISA KIT; vWF-cleaving protease ELISA KIT
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Catalog
E040443
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species
Human
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GenevWFCP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor Cleaving Protease (vWFCP).
No significant cross-reactivity or interference between Von Willebrand Factor Cleaving Protease (vWFCP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 26.2pg/mL.
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Intended UseHuman vWFCP ELISA Kit allows for the in vitro quantitative determination of vWFCP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Willebrand Factor Cleaving Protease (vWFCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Von Willebrand Factor Cleaving Protease (vWFCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Willebrand Factor Cleaving Protease (vWFCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor Cleaving Protease (vWFCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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