Human VWA3A ELISA Kit

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  • Alternative name

    Human von Willebrand factor A domain containing 3A ELISA Kit;Human von Willebrand factor A domain-containing protein 3A ELISA Kit;

  • Catalog
    E040437
  • species
    Human
  • GeneVWA3A
  • Standard CurveHuman VWA3A ELISA Kit
  • Other Species Mouse Vwa3a ELISA KitRat Vwa3a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of vWA3A. No significant cross-reactivity or interference between vWA3A and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of vWA3A is typically less than 0.15ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseHuman VWA3A ELISA Kit allows for the in vitro quantitative determination of VWA3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWA3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to vWA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain vWA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of vWA3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human von Willebrand factor A domain-containing protein 3A Protein information
  • Uniprot ID VWA3A_HUMAN
  • Uniprot AC A6NCI4; A4QMU8; A6NNC0; Q6UTX4; Q6ZQZ9; Q8IUY6; Q8N9W1;
  • UniGene Hs.10697;
  • GeneID 146177
  • KEGG hsa:146177;
  • Human von Willebrand factor A domain-containing protein 3A Protein SEQUENCE
  • SEQUENCE 1184 AA; 134020 MW; 1CA57332FA8879F0 CRC64;

    MKKYRKISIG CFAMATQTSH VFHGQENMFL ENHCIRRNTG RDSKKPLKQK

    NMNGLGQNSD NGLLVTHVNQ TQDLLRLQGS ETQSSDWEDS EDWLSAHSLK

    CQKLTLADLI SQGTEVLEEG TNVVQKICFS TQIIRHFESK LSDTIEVYQE

    RIQWLTENSK KAFGLIKGAR VSILIDVSAI SSGPQKEEFQ KDLMSLIDEQ

    LSHKEKLFVL SFGTNAGSLW PDPMEVSAST LQELKLWVKT LQPDGGSNLL

    QALKKIFTLK GLDSLVAIMR SCPDQPSEIL SDYIQQSTMG RDLIIHFITY

    RCDDQMPPAV LKNLAEAVRG YYHCYSPKME HYTSRDMDEL LAEIQKAQSL

    LSHVQALQHS SPCEALTCTM EEISTEITNG PLISLLPKPP KHDAPLTIEF

    PNLDKTSAEW LKVNGLKAKK LSLYQVLAPN AFSPVEEFVP ILQKTVSSTI

    HEKAMIQFEW HDGTVKNIHV DPPFLYKYQQ QLSRAMRMYE RRIEWLSLAS

    RRIWGTVCEK RVVVLLDISA TNSMYIIHIQ HSLRLLLEEQ LSNKDCFNLI

    AFGSTIESWR PEMVPVSHNN LQSAWRWALN LRCRGSRNVL SALRKAVEVD

    FKDKDKHQSQ GIYLFTGGIP DQDMPTLSAY MAEACGGCDL QLNVCLFYVG

    EPKMDTTPPA RYASHTDTAA AYKEVTRAAG GRFHWFGDTG IYESDDINSI

    MSEMEKALNY SQKCAFLMAS LKNHSGKVLG SSALPKEKPK TLQLRSQPKK

    LCPPRPTVPL GARMSIKDDP DREKSPPLKS LKWRPLSSRV GISPAAAQPT

    KEGMMELRRK TKSREAETSL LLFYTEKGND VGSVYKKYPQ GRGLRRTSSS

    IDLPRKDTVC SSQEWVAKYG LKKLKLEISR CMGPNCTHQK SGQRSASAKH

    CSIFPSVEIH GVVRHIQWTP REMEVYIRHL EKVLRRYVQR LQWLLSGSRR

    LFGTVLESKV CILLDTSGSM GPYLQQVKTE LVLLIWEQLR KCCDSFNLLS

    FAESFQSWQD TLVETTDAAC HEAMQWVTHL QAQGSTSILQ ALLKAFSFHD

    LEGLYLLTDG KPDTSCSLVL NEVQKLREKR DVKVHTISLN CSDRAAVEFL

    RKLASFTGGR YHCPVGEDTL SKIHSLLTKG FINEKDPTLP PFEGDDLRIL

    AQEITKARSF LWQAQSFRSQ LQKKNDAEPK VTLS

  • UCSC uc010bxe.2; human. [A6NCI4-1];



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