Human VWA3A ELISA Kit

Alternative name
Human von Willebrand factor A domain containing 3A ELISA Kit;Human von Willebrand factor A domain-containing protein 3A ELISA Kit;
Catalog
E040437
species
Human
GeneVWA3A
SpecificityThis assay has high sensitivity and excellent specificity for detection of vWA3A. No significant cross-reactivity or interference between vWA3A and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of vWA3A is typically less than 0.15ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseHuman VWA3A ELISA Kit allows for the in vitro quantitative determination of VWA3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWA3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to vWA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain vWA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of vWA3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human von Willebrand factor A domain-containing protein 3A Protein information
Uniprot ID VWA3A_HUMAN
Uniprot AC A6NCI4; A4QMU8; A6NNC0; Q6UTX4; Q6ZQZ9; Q8IUY6; Q8N9W1;
UniGene Hs.10697;
GeneID 146177
KEGG hsa:146177;
Human von Willebrand factor A domain-containing protein 3A Protein SEQUENCE
SEQUENCE 1184 AA; 134020 MW; 1CA57332FA8879F0 CRC64;
MKKYRKISIG CFAMATQTSH VFHGQENMFL ENHCIRRNTG RDSKKPLKQK
NMNGLGQNSD NGLLVTHVNQ TQDLLRLQGS ETQSSDWEDS EDWLSAHSLK
CQKLTLADLI SQGTEVLEEG TNVVQKICFS TQIIRHFESK LSDTIEVYQE
RIQWLTENSK KAFGLIKGAR VSILIDVSAI SSGPQKEEFQ KDLMSLIDEQ
LSHKEKLFVL SFGTNAGSLW PDPMEVSAST LQELKLWVKT LQPDGGSNLL
QALKKIFTLK GLDSLVAIMR SCPDQPSEIL SDYIQQSTMG RDLIIHFITY
RCDDQMPPAV LKNLAEAVRG YYHCYSPKME HYTSRDMDEL LAEIQKAQSL
LSHVQALQHS SPCEALTCTM EEISTEITNG PLISLLPKPP KHDAPLTIEF
PNLDKTSAEW LKVNGLKAKK LSLYQVLAPN AFSPVEEFVP ILQKTVSSTI
HEKAMIQFEW HDGTVKNIHV DPPFLYKYQQ QLSRAMRMYE RRIEWLSLAS
RRIWGTVCEK RVVVLLDISA TNSMYIIHIQ HSLRLLLEEQ LSNKDCFNLI
AFGSTIESWR PEMVPVSHNN LQSAWRWALN LRCRGSRNVL SALRKAVEVD
FKDKDKHQSQ GIYLFTGGIP DQDMPTLSAY MAEACGGCDL QLNVCLFYVG
EPKMDTTPPA RYASHTDTAA AYKEVTRAAG GRFHWFGDTG IYESDDINSI
MSEMEKALNY SQKCAFLMAS LKNHSGKVLG SSALPKEKPK TLQLRSQPKK
LCPPRPTVPL GARMSIKDDP DREKSPPLKS LKWRPLSSRV GISPAAAQPT
KEGMMELRRK TKSREAETSL LLFYTEKGND VGSVYKKYPQ GRGLRRTSSS
IDLPRKDTVC SSQEWVAKYG LKKLKLEISR CMGPNCTHQK SGQRSASAKH
CSIFPSVEIH GVVRHIQWTP REMEVYIRHL EKVLRRYVQR LQWLLSGSRR
LFGTVLESKV CILLDTSGSM GPYLQQVKTE LVLLIWEQLR KCCDSFNLLS
FAESFQSWQD TLVETTDAAC HEAMQWVTHL QAQGSTSILQ ALLKAFSFHD
LEGLYLLTDG KPDTSCSLVL NEVQKLREKR DVKVHTISLN CSDRAAVEFL
RKLASFTGGR YHCPVGEDTL SKIHSLLTKG FINEKDPTLP PFEGDDLRIL
AQEITKARSF LWQAQSFRSQ LQKKNDAEPK VTLS
UCSC uc010bxe.2; human. [A6NCI4-1];

Category

Human VWA3A ELISA Kit

Human von Willebrand factor A domain containing 3A ELISA Kit;Human von Willebrand factor A domain-containing protein 3A ELISA Kit;