Human ACTG2 ELISA Kit

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  • Alternative name

    Human alpha-actin-3 ELISA Kit;Human Gamma-2-actin ELISA Kit;Human Smooth muscle gamma-actin ELISA Kit;Human ACTA3 ELISA Kit;Human ACTL3 ELISA Kit;Human ACTSG ELISA Kit;Human ACT ELISA Kit;Human ACTE ELISA Kit;Human VSCM ELISA Kit;Human actin, gamma 2, smooth muscle, enteric ELISA Kit;Human actin, gamma-enteric smooth muscle ELISA Kit;Human actin-like protein ELISA Kit;

  • Catalog
    E000404
  • species
    Human
  • GeneACTG2
  • Standard CurveHuman ACTG2 ELISA Kit
  • Other Species Mouse Actg2 ELISA KitBovine ACTG2 ELISA KitChicken ACTG2 ELISA KitRat Actg2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Actin Gamma 2, Smooth Muscle (ACTg2). No significant cross-reactivity or interference between Actin Gamma 2, Smooth Muscle (ACTg2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman ACTG2 ELISA Kit allows for the in vitro quantitative determination of ACTG2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Gamma 2, Smooth Muscle (ACTg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Gamma 2, Smooth Muscle (ACTg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Actin, gamma-enteric smooth muscle Protein information
  • Uniprot ID ACTH_HUMAN
  • Uniprot AC P63267; B2R7E7; B4E315; D6W5H8; E9PG30; P12718; Q504R1; Q6FI22;
  • UniGene Hs.516105;
  • GeneID 72
  • KEGG hsa:72;
  • Human Actin, gamma-enteric smooth muscle Protein SEQUENCE
  • SEQUENCE 376 AA; 41877 MW; 6EC08CD5EEAD445E CRC64;

    MCEEETTALV CDNGSGLCKA GFAGDDAPRA VFPSIVGRPR HQGVMVGMGQ

    KDSYVGDEAQ SKRGILTLKY PIEHGIITNW DDMEKIWHHS FYNELRVAPE

    EHPTLLTEAP LNPKANREKM TQIMFETFNV PAMYVAIQAV LSLYASGRTT

    GIVLDSGDGV THNVPIYEGY ALPHAIMRLD LAGRDLTDYL MKILTERGYS

    FVTTAEREIV RDIKEKLCYV ALDFENEMAT AASSSSLEKS YELPDGQVIT

    IGNERFRCPE TLFQPSFIGM ESAGIHETTY NSIMKCDIDI RKDLYANNVL

    SGGTTMYPGI ADRMQKEITA LAPSTMKIKI IAPPERKYSV WIGGSILASL

    STFQQMWISK PEYDEAGPSI VHRKCF

  • UCSC uc002sjw.4; human. [P63267-1];




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