Intended UseHuman VKORC1 ELISA Kit allows for the in vitro quantitative determination of VKORC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-VKOR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VKOR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VKOR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VKOR1 can be calculated.
Human Vitamin K epoxide reductase complex subunit 1 Protein information
Uniprot ID
VKOR1_HUMAN
Uniprot AC
Q9BQB6; A6NIQ6; B2R4Z6; Q6UX90; Q7Z2R4;
UniGene
Hs.324844;
GeneID
79001
KEGG
hsa:79001;
Human Vitamin K epoxide reductase complex subunit 1 Protein SEQUENCE