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Human DDX42 ELISA Kit

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  • Alternative name

    Human DEAD box protein 42 ELISA Kit;Human RNA helicase-like protein ELISA Kit;Human RHELP ELISA Kit;Human RNA helicase-related protein ELISA Kit;Human RNAHP ELISA Kit;Human SF3b DEAD box protein ELISA Kit;Human splicing factor 3B-associated 125 kDa protein ELISA Kit;Human SF3b125 ELISA Kit;Human DDX42P ELISA Kit;Human SF3B8 ELISA Kit;Human DEAD-box helicase 42 ELISA Kit;Human ATP-dependent RNA helicase DDX42 ELISA Kit;Human DEAD (Asp-Glu-Ala-Asp) box helicase 42 ELISA Kit;Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 ELISA Kit;Human splicing factor 3b, subunit 8 ELISA Kit;
  • Catalog
    E004034
  • species
    Human
  • GeneDDX42
  • Standard CurveHuman DDX42 ELISA Kit
  • Other Species Mouse Ddx42 ELISA KitChicken DDX42 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DDX42. No significant cross-reactivity or interference between DDX42 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDX42 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DDX42 ELISA Kit allows for the in vitro quantitative determination of DDX42 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Intended Uses: This DDX42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DDX42. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||DDX42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDX42 antibody and an DDX42-HRP conjugate. The assay sample and buffer are incubated together with DDX42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX42 concentration since DDX42 from samples and DDX42-HRP conjugate compete for the anti-DDX42 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX42 from the sample, fewer sites are left to bind DDX42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX42 concentration in each sample is interpolated from this standard curve.
  • Human ATP-dependent RNA helicase DDX42 Protein information
  • Uniprot ID DDX42_HUMAN
  • Uniprot AC Q86XP3; A6NML1; A8KA43; O75619; Q68G51; Q96BK1; Q96HR7; Q9Y3V8;
  • UniGene Hs.702010;
  • GeneID 11325
  • KEGG hsa:11325;
  • Human ATP-dependent RNA helicase DDX42 Protein SEQUENCE

  • SEQUENCE 938 AA; 102975 MW; D89A252E095D8913 CRC64;

    MNWNKGGPGT KRGFGFGGFA ISAGKKEEPK LPQQSHSAFG ATSSSSGFGK

    SAPPQLPSFY KIGSKRANFD EENAYFEDEE EDSSNVDLPY IPAENSPTRQ

    QFHSKPVDSD SDDDPLEAFM AEVEDQAARD MKRLEEKDKE RKNVKGIRDD

    IEEEDDQEAY FRYMAENPTA GVVQEEEEDN LEYDSDGNPI APTKKIIDPL

    PPIDHSEIDY PPFEKNFYNE HEEITNLTPQ QLIDLRHKLN LRVSGAAPPR

    PGSSFAHFGF DEQLMHQIRK SEYTQPTPIQ CQGVPVALSG RDMIGIAKTG

    SGKTAAFIWP MLIHIMDQKE LEPGDGPIAV IVCPTRELCQ QIHAECKRFG

    KAYNLRSVAV YGGGSMWEQA KALQEGAEIV VCTPGRLIDH VKKKATNLQR

    VSYLVFDEAD RMFDMGFEYQ VRSIASHVRP DRQTLLFSAT FRKKIEKLAR

    DILIDPIRVV QGDIGEANED VTQIVEILHS GPSKWNWLTR RLVEFTSSGS

    VLLFVTKKAN AEELANNLKQ EGHNLGLLHG DMDQSERNKV ISDFKKKDIP

    VLVATDVAAR GLDIPSIKTV INYDVARDID THTHRIGRTG RAGEKGVAYT

    LLTPKDSNFA GDLVRNLEGA NQHVSKELLD LAMQNAWFRK SRFKGGKGKK

    LNIGGGGLGY RERPGLGSEN MDRGNNNVMS NYEAYKPSTG AMGDRLTAMK

    AAFQSQYKSH FVAASLSNQK AGSSAAGASG WTSAGSLNSV PTNSAQQGHN

    SPDSPVTSAA KGIPGFGNTG NISGAPVTYP SAGAQGVNNT ASGNNSREGT

    GGSNGKRERY TENRGSSRHS HGETGNRHSD SPRHGDGGRH GDGYRHPESS

    SRHTDGHRHG ENRHGGSAGR HGENRGANDG RNGESRKEAF NRESKMEPKM

    EPKVDSSKMD KVDSKTDKTA DGFAVPEPPK RKKSRWDS

  • UCSC uc002jbu.5; human. [Q86XP3-1];



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