Human BAP1 ELISA Kit

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  • Alternative name

    Human BRCA1-associated protein 1 ELISA Kit;Human cerebral protein 6 ELISA Kit;Human KIAA0272 ELISA Kit;Human HUCEP-13 ELISA Kit;Human UCHL2 ELISA Kit;Human hucep-6 ELISA Kit;Human BRCA1 associated protein 1 ELISA Kit;Human ubiquitin carboxyl-terminal hydrolase BAP1 ELISA Kit;Human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) ELISA Kit;Human cerebral protein-13 ELISA Kit;

  • Catalog
    E040264
  • species
    Human
  • GeneBAP1
  • Standard CurveHuman BAP1 ELISA Kit
  • Other Species Mouse BAP1 ELISA KitBovine BAP1 ELISA KitChicken BAP1 ELISA KitRat Bap1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BAP1. No significant cross-reactivity or interference between BAP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BAP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman BAP1 ELISA Kit allows for the in vitro quantitative determination of BAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: BAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BAP1 antibody and an BAP1-HRP conjugate. The assay sample and buffer are incubated together with BAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAP1 concentration since BAP1 from samples and BAP1-HRP conjugate compete for the anti-BAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BAP1 from the sample, fewer sites are left to bind BAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAP1 concentration in each sample is interpolated from this standard curve.
  • Human Ubiquitin carboxyl-terminal hydrolase BAP1 Protein information
  • Uniprot ID BAP1_HUMAN
  • Uniprot AC Q92560; A8K993; Q6LEM0; Q7Z5E8;
  • UniGene Hs.106674;
  • GeneID 8314
  • KEGG hsa:8314;
  • Human Ubiquitin carboxyl-terminal hydrolase BAP1 Protein SEQUENCE
  • SEQUENCE 729 AA; 80362 MW; 031DA03AE1841D85 CRC64;

    MNKGWLELES DPGLFTLLVE DFGVKGVQVE EIYDLQSKCQ GPVYGFIFLF

    KWIEERRSRR KVSTLVDDTS VIDDDIVNNM FFAHQLIPNS CATHALLSVL

    LNCSSVDLGP TLSRMKDFTK GFSPESKGYA IGNAPELAKA HNSHARPEPR

    HLPEKQNGLS AVRTMEAFHF VSYVPITGRL FELDGLKVYP IDHGPWGEDE

    EWTDKARRVI MERIGLATAG EPYHDIRFNL MAVVPDRRIK YEARLHVLKV

    NRQTVLEALQ QLIRVTQPEL IQTHKSQESQ LPEESKSASN KSPLVLEANR

    APAASEGNHT DGAEEAAGSC AQAPSHSPPN KPKLVVKPPG SSLNGVHPNP

    TPIVQRLPAF LDNHNYAKSP MQEEEDLAAG VGRSRVPVRP PQQYSDDEDD

    YEDDEEDDVQ NTNSALRYKG KGTGKPGALS GSADGQLSVL QPNTINVLAE

    KLKESQKDLS IPLSIKTSSG AGSPAVAVPT HSQPSPTPSN ESTDTASEIG

    SAFNSPLRSP IRSANPTRPS SPVTSHISKV LFGEDDSLLR VDCIRYNRAV

    RDLGPVISTG LLHLAEDGVL SPLALTEGGK GSSPSIRPIQ GSQGSSSPVE

    KEVVEATDSR EKTGMVRPGE PLSGEKYSPK ELLALLKCVE AEIANYEACL

    KEEVEKRKKF KIDDQRRTHN YDEFICTFIS MLAQEGMLAN LVEQNISVRR

    RQGVSIGRLH KQRKPDRRKR SRPYKAKRQ

  • UCSC uc003ddx.5; human.;


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