Human Ub ELISA Kit

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  • Alternative name

    Polyubiquitin-C ELISA KIT; UBC ELISA KIT;

  • Catalog
    E040242
  • species
    Human
  • GeneUb
  • Standard CurveHuman Ub ELISA Kit
  • Other Species Mouse Ub ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ubiquitin (Ub) No significant cross-reactivity or interference between Ubiquitin (Ub) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 25.4pg/mL.
  • Intended UseHuman Ub ELISA Kit allows for the in vitro quantitative determination of Ub , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin (Ub). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin (Ub). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin (Ub), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin (Ub) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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