Human (E3-independent) E2 ubiquitin-conjugating enzyme C ELISA Kit;Human E2 ubiquitin-conjugating enzyme C ELISA Kit;Human UBCH10 ELISA Kit;Human Ubiquitin carrier protein C ELISA Kit;Human ubiquitin-protein ligase C ELISA Kit;Human dJ447F3.2 ELISA Kit;Human ubiquitin conjugating enzyme E2 C ELISA Kit;Human ubiquitin-conjugating enzyme E2 C ELISA Kit;Human cyclin-selective ubiquitin carrier protein ELISA Kit;Human mitotic-specific ubiquitin-conjugating enzyme ELISA Kit;Human ubiquitin conjugating enzyme E2C ELISA Kit;
Catalog
E040210
species
Human
GeneUBE2C
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human UBE2C. No significant cross-reactivity or interference between Human UBE2C and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human UBE2C and all the analogues, therefore, cross reaction may still exist.
Intended UseHuman UBE2C ELISA Kit allows for the in vitro quantitative determination of UBE2C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of UBE2C in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UBE2C. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human UBE2C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE2C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human UBE2C. You can calculate the concentration of Human UBE2C in the samples by comparing the OD of the samples to the standard curve.
Human Ubiquitin-conjugating enzyme E2 C Protein information
Uniprot ID
UBE2C_HUMAN
Uniprot AC
O00762; A6NP33; E1P5N7; G3XAB7;
UniGene
Hs.93002;
GeneID
11065
KEGG
hsa:11065;
Human Ubiquitin-conjugating enzyme E2 C Protein SEQUENCE