Human DIO3 ELISA Kit

Alternative name
Human 5DIII ELISA Kit;Human DIOIII ELISA Kit;Human type 3 DI ELISA Kit;Human Type III iodothyronine deiodinase ELISA Kit;Human ITDI3 ELISA Kit;Human TXDI3 ELISA Kit;Human D3 ELISA Kit;Human iodothyronine deiodinase 3 ELISA Kit;Human thyroxine 5-deiodinase ELISA Kit;Human deiodinase, iodothyronine type III ELISA Kit;Human iodothyronine deiodinase, placental type ELISA Kit;Human thyroxine deiodinase type III (selenoprotein) ELISA Kit;Human type 3 iodothyronine selenodeiodinase ELISA Kit;Human type-III 5' deiodinase ELISA Kit;
Catalog
E040106
species
Human
GeneDIO3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman DIO3 ELISA Kit allows for the in vitro quantitative determination of DIO3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human DIO3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DIO3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DIO3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DIO3 can be calculated.
Human Thyroxine 5-deiodinase Protein information
Uniprot ID IOD3_HUMAN
Uniprot AC P55073; G3XAM0; Q8WVN5;
UniGene Hs.49322;
GeneID 1735
KEGG hsa:1735;
Human Thyroxine 5-deiodinase Protein SEQUENCE
SEQUENCE 304 AA; 33947 MW; 08B61835B353D3C7 CRC64;
MPRQATSRLV VGEGEGSQGA SGPAATMLRS LLLHSLRLCA QTASCLVLFP
RFLGTAFMLW LLDFLCIRKH FLGRRRRGQP EPEVELNSEG EEVPPDDPPI
CVSDDNRLCT LASLKAVWHG QKLDFFKQAH EGGPAPNSEV VLPDGFQSQH
ILDYAQGNRP LVLNFGSCTU PPFMARMSAF QRLVTKYQRD VDFLIIYIEE
AHPSDGWVTT DSPYIIPQHR SLEDRVSAAR VLQQGAPGCA LVLDTMANSS
SSAYGAYFER LYVIQSGTIM YQGGRGPDGY QVSELRTWLE RYDEQLHGAR
PRRV
UCSC uc021sdx.1; human.;

Human DIO3 ELISA Kit

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