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Alternative name
Tumor necrosis factor receptor superfamily member 9 ELISA KIT; 4-1BB ligand receptor ELISA KIT; CDw137 ELISA KIT; T-cell antigen 4-1BB homolog ELISA KIT; T-cell antigen ILA ELISA KIT; CD_antigen: CD137TNFRSF9 ELISA KIT; CD137 ELISA KIT; ILA ELISA KIT
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Catalog
E039963
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species
Human
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GeneTNFRSF9/4-1BB
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<18.75pg/ml
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Intended UseHuman TNFRSF9/4-1BB ELISA Kit allows for the in vitro quantitative determination of TNFRSF9/4-1BB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human 4- 1BB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human 4-1BB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human 4-1BB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human 4-1BBcan be calculated.
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