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Alternative name
ATP-binding cassette sub-family C member 11 ELISA KIT; Multidrug resistance-associated protein 8ABCC11 ELISA KIT; MRP8 ELISA KIT
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Catalog
E003996
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species
Human
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GeneABCC11
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Standard Curve
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Other Species
Mouse ABCC11 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C11 (ABCC11).
No significant cross-reactivity or interference between ATP Binding Cassette Transporter C11 (ABCC11) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.103ng/mL.
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Intended UseHuman ABCC11 ELISA Kit allows for the in vitro quantitative determination of ABCC11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C11 (ABCC11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C11 (ABCC11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C11 (ABCC11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C11 (ABCC11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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