Intended UseHuman TNFRSF12A ELISA Kit allows for the in vitro quantitative determination of TNFRSF12A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human TNFRSF12A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TNFRSF12A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFRSF12A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TNFRSF12A can be calculated.
Human Tumor necrosis factor receptor superfamily member 12A Protein information
Q9NP84; D3DUA6; Q9HCS0;
Human Tumor necrosis factor receptor superfamily member 12A Protein SEQUENCE