Human TNFSF11/RANKL ELISA Kit

Contact Us
  • Alternative name

    Tumor necrosis factor ligand superfamily member 11 ELISA KIT; Osteoclast differentiation factor ELISA KIT; ODFTNFSF11 ELISA KIT; OPGL ELISA KIT; RANKL ELISA KIT; TRANCE ELISA KIT; ODF ELISA KIT; OPGL ELISA KIT; RANKL ELISA KIT; TRANCE ELISA KIT

  • Catalog
    E039901
  • species
    Human
  • GeneTNFSF11/RANKL
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman TNFSF11/RANKL ELISA Kit allows for the in vitro quantitative determination of TNFSF11/RANKL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RANKL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RANKL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RANKL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RANKLcan be calculated.




tag

Inquiry About Human TNFSF11/RANKL ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!