SpecificityThis assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Beta (TNFb).
No significant cross-reactivity or interference between Tumor Necrosis Factor Beta (TNFb) and analogues was observed.
Intended UseHuman TNFb ELISA Kit allows for the in vitro quantitative determination of TNFb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Beta (TNFb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Beta (TNFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Beta (TNFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Beta (TNFb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.